碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 102 / Squid is important catches in Taiwan's offshore fishing. However, squid viscera and ink sac in the fish processing industry are discarded or as byproducts. Among them, squid ink sac (SIS) has fish stench and is difficult to utilize. In this study, the proximate compositions analysis and deodorant treatments of SIS from neon flying squid (Ommastrephes bartramii) were investigated. The proximate compositions in SIS were moisture: 62.64%, crude protein: 28.54%, other: 7.02%, ash: 1.81% and crude fat: 0.51%. The squid ink powder products (SIP) by deodorant treatments including no treatment (control), ethanol or hexane treatments, different alkaline concentration treatments (High, Medium, Low), or combination of ethanol or hexane solvents and alkaline solution treatments were analyzed for deodorant evaluation. The values of yield (%) and total volatile basic nitrogen (TVBN) of deodorant SIPs decreased with increased alkaline concentration in alkaline groups or combination groups, while the high alkaline group has the lowest yield (11.28%) and TVBN (9.99 mg/100g). In color measurement, L* values (14.23-17.61) of all treated groups were higher than that of the control group (12.94), whereas the blackness (87.14) in the control group is the highest one. Solubility of all group (including control) onto water, 1N NaOH and different acidity solution were are less than 14.39%, 17.13% and 18.98%, respectively. In above-mentioned results, the SIP of medium alkaline concentration treatment had the highest score of sensory acceptance and its deodorant result was more effective.
Moreover, the antioxidant and antibacterial effects of SIS extracts extracted by methanol, ethanol, hexane and acetone as well as SIS polysaccharide were evaluated. Except for ferric reducing power, the SIS polysaccharide showed higher antioxidant ability in scavenging DPPH (EC50=0.393 mg/mL), ABTS radical scavenging (EC50=0.257 mg/mL), ferrous ion-chelating ability (EC50=0.282 mg/mL) and hydroxyl groups scavenging ability (EC50=0.397 mg/mL) than other SIS extracts extracted by four organic solvents. In antibacterial effect, significant inhibition zones were observed for methanol and ethanol extracts, and polysaccharide of SIS at 200 mg/mL against pathogenic bacteria (Staphylococcus aureus, Salmonella Typhimurium, Escherichia coli, Vibrio parahaemolyticus) and histamine-producing bacteria (Morganella morganii, Raoultella ornithinolytica) by agar well diffusion method. The minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) of the SIS polysaccharide were the lowest and less than 25 mg/mL against above bacterial strains. The SIS polysaccharide showed the best antibacterial effect for V. parahaemolyticus, and the MIC and MBC were 6.25 mg/mL and 12.5 mg/mL, respectively. Therefore, the results showed that SIS polysaccharide has excellent antioxidant and antibacterial activities.
| Identifer | oai:union.ndltd.org:TW/102NKIM0084013 |
| Date | January 2014 |
| Creators | Kai-Yuan Jheng, 鄭凱元 |
| Contributors | 蔡永祥教授 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 112 |
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