碩士 / 國立高雄海洋科技大學 / 海洋生物技術研究所 / 102 / Taiwan, a country renowned for its world-leading production of tiger prawns, has unfortunately been struck with white spot syndrome (WSS), severely reducing production. As the disease is currently incurable, developing a vaccine has become a top priority. This research sought to use probiotics to transport the envelope protein VP28 of the white spot syndrome virus (WSSV) as a type of vaccine. First, the characteristics of Pseudomonas sp. were analyzed, and the results showed that a salinity level of 0%~4% is suitable for the growth of Pseudomonas sp. and that its nitrite degradation ability is better than that of Bacillus sp. VP28 gene was cloned into pBBR1MCS-2 which does not require IPTG to induce, and then used electroporation to transform pMCS-VP28 into Pseudomonas sp. Two days after administering the VP28 vaccine to tiger prawns, VP28 was found in their intestines, and it was also found in their muscles after 4 days. A WSSV virus diluted to 5x105 was then used in the challenge tests. The 100ppm and 200ppm vaccine groups exhibited additional survival rates of 10% and 20%, respectively, compared to the control group. According to the immune parameter analysis, the haemocyte count, prophenoloxidase (proPO) activity, and phagocytosis ability of the vaccinated groups were all higher than those of the control group. This experiment verified that using the VP28 vaccine can improve the survival rate as well as the immunity of tiger prawns. After altering its medium, low-cost mass production of the vaccine can commence, allowing for widespread application in the future. Furthermore, it is hoped that by using Sia10, the vaccine can be converted into a secretable form for which additional field testing will be conducted.
| Identifer | oai:union.ndltd.org:TW/102NKIM0270008 |
| Date | January 2014 |
| Creators | Lin,Si-Wei, 林思維 |
| Contributors | Huang,Ying-Tang, 黃胤唐 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 81 |
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