碩士 / 國立屏東科技大學 / 水產養殖系所 / 102 / An experiment was conducted to evaluate the potential of a Bacillus subtilis E20-fermented mixture (FSFEM) containing soybean meal (SBM) and Eisenia fetida earthworm meal (EM) at a ratio of 4:1 to increase the methionine level in order to satisfy the methionine requirement of white shrimp, Litopenaeus vannamei, in a diet with fish meal completely replaced by mixtures. The amount of initial moisture influences the fermentation status. Protein content and B. subtilis E20 proliferation were significantly increased in the fermented mixture when initial moisture was 30-50%. B. subtilis E20 fermentation improved the mixture’s palatability and utilization based on better growth performance in comparison to shrimp fed FSEM (containing fermented SBM and EM at a ratio of 4:1). FSFEM is good substitute for fish meal. Maximal replacement levels of fish meal with FSFEM were 80% in a shrimp diet with 37% of crude protein and 7% of crude lipid based on the growth performance and 100% based on feeding efficiency. In addition, shrimp fed experimental diets had no significant differences in survival after being challenged by Vibrio alginolyticus. It is suggested that B. subtilis E20-FSFEM has the potential to replace fish meal in cultured shrimp diets.
| Identifer | oai:union.ndltd.org:TW/102NPUS5086003 |
| Date | January 2014 |
| Creators | Guei, Wan-Chen, 貴萬真 |
| Contributors | Liu, Chun-Hun, 劉俊宏 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 65 |
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