碩士 / 國立中山大學 / 生物科學系研究所 / 102 / Genetic programs and signaling pathways are required for proper growth and patterning of blood vessels, however, little known about the transcription factors functioning in vein identity and intersegmental vessels (ISV) patterning in zebrafish.
The orphan nuclear receptor Chicken ovalbumin upstream promoter transcription factor II (CoupTFII) positively regulates vein identity in mice. Here we show that the CoupTF1b is important for vein and tip cell identity. Comparison of amino acids sequence and phylogenetic analysis of CoupTF1b orthologs in different species suggesting the CoupTF1b is conserved among vertebrates. CoupTF1b mRNA has a spatiotemporal expression pattern in ventral lateral mesoderm consistent with a role in early vascular specification. Morpholino
knockdown of coupTFIb results in a decrease in both venous fluorescent signals and expression of the vein specific marker flt4 and mrc1. These data suggest that CoupTF1b has novel role in promoting vein identity. In addition, we show loss of CoupTF1b impairs ISV growth, suggesting that CoupTF1b also has an important role in controlling ISV growth. We have confirmed those vascular defects are CoupTF1b specific by using two different types of morpholinos knockdown strategies (ATG and splicing morpholinos). To address whether the decrease in vein signals and the growth defect in ISV result from cell death, we performed TUNEL assay. We showed that an increase in non-specific cell death after morpholino injection is possible cause of the observed vascular phenotype. We further test CoupTF1b likely interact with the Notch signaling pathway to control vascular development. Together, we show CoupTF1b plays an indispensable role for vascular development in zebrafish.
| Identifer | oai:union.ndltd.org:TW/102NSYS5112024 |
| Date | January 2014 |
| Creators | Yu-Zheng Mou, 牟育正 |
| Contributors | Chang-Yi, Wu, 吳長益 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 78 |
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