碩士 / 國立中山大學 / 海洋科學系研究所 / 102 / Carotenoids occupy an important niche in current natural pigments market. They can be used as feed additive, food colorants, cosmeceutical or dietary supplement.. The application and effect of astaxanthin are actively studied due to its high antioxidant capacity in recent years. A new species of thermotolerant microalga was isolated in tropical Taiwan, and named as Coelastrella sp. F50. These cells produce reddish pigments under environmental stress. The pigments contain astaxantin, canthaxanthin, lutein and beta-carotene analyzed using high performance liquid chromatography. Productivity of astaxanthin in this species was investigated. Optimization of saponification conditions demonstarate that 10 mM NaOH mM was the optimal concentration to react for two hours. Optimization of irradiance stress shows that 800 μmol/m2s leads to the highest astaxanthin accumulation in the cultures. Bicarbonate (NaHCO3) enhanced astaxanthin accumulation, and the level of 2 g/L had the best effect. In 14 days of cultivation, biomass of the cells increase from initial 100 mg/L to 2 g/L and maximum astaxanthin content reached 42 mg/L (2.1%). The maximum astaxanthin productivity reached 3 to 15 mg/L/day depending on different cultivation scenarios. Based on the results, Coelastrella sp. F50 has a chance to commercially produce natural astaxanthin in tropical areas. More studies are needed to further optimize astaxanthin production using this new microalga.
| Identifer | oai:union.ndltd.org:TW/102NSYS5276013 |
| Date | January 2014 |
| Creators | Po-Chien Yu, 尤柏謙 |
| Contributors | Ching-Nen Nathan Chen, 陳慶能 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 51 |
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