碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 102 / New bioactive compounds in microalgae increase their commercial values for healthy food. Nannochloropsis oculata is mostly used for juvenile feed, while application for health is unavailable so this research isolated the compositions of the anti-inflammatory ingredients from N. oculata. The results showed growth of N. oculata yielded well in 110 rpm, 25 oC, 15000 lux, and 2.8% salinity; additional nutrients and CaCO3 slightly increased production. The analysis of N. oculata anti-inflammatory ingredients was based on the inhibition ratios of iNOS and COX-2 protein expression in RAW 264.7 under LPS induction. TLC separation showed that highly polar molecules inhibited iNOS and COX-2 expressions. AWP2-11-1 and AWP2-11-3 compounds were from acetone partition. In RP-C18 separation of the extracts, A3-19-21 compound inhibited iNOS and COX-2 expressions by 99% and 95%, respectively. HP2-69-70 compound from n-hexane partition inhibited iNOS expression by 64%. The resulting compounds were identified for their structures via 1H-NMR, 13C-NMR, 2 D NMR, and mass spectrometries.
| Identifer | oai:union.ndltd.org:TW/102NSYS5277014 |
| Date | January 2014 |
| Creators | Chi-Jui Lin, 林奇叡 |
| Contributors | Ping-Jyun Sung, Chan-Shing Lin, 宋秉鈞, 林全信 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 143 |
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