碩士 / 國立臺灣師範大學 / 生命科學研究所 / 102 / Under temperature perturbations, metabolic strategies of ectothermic vertebrates would be modified and further increase the cellular reactive oxygen/nitrogen species (ROS/RNS) formations. Previous studies indicated that excess ROS/RNS accumulation would cause cytotoxicity. However in cephalopods, the cellular and physiological mechanisms behind metabolic adaptation under temperature perturbations is still an open question. In this study, we use squid (S. lessoniana) embryos as a cephalopod model to investigate the antioxidant mechanisms and further metabolic modifications under hyperthermic environment. According to oxygen consumption rate and ammonium excretion rate studies in squid embryos, 25-stage squid embryos were sensitive to ambient hyperthermic stress and further selected for following experiments. Under hyperthermic conditions, intracellular ROS/RNS contents were not obviously changed. Transcripts expressions of cytochrome c oxidase I (Cox I), anti-oxidation molecules (CAT, SOD, HRP) and stress-resistant genes (HSP70, HSP90) were up-regulated under hyperthermic stress. Moreover protein expressions of hypoxia-inducible factor 1-alpha (HIF-1) and Na+-K+-ATPase (NKA) are close related to cellular adaptive metabolic shift in hypothermic conditions. In conclusion, intact metabolic rate increment in squid embryos under hyperthermic stress would further induce adaptive metabolism shift for proper physiological functions and avoiding ROS/RNS accumulation.
keywords：hyperthermic environment、Sepioteuthis lessoniana、oxidative metabolism
| Identifer | oai:union.ndltd.org:TW/102NTNU5112021 |
| Date | January 2014 |
| Creators | Pou-Long Kuan, 關寶龍 |
| Contributors | Yung-Che Tseng, 曾庸哲 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 49 |
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