碩士 / 國立臺灣師範大學 / 生命科學研究所 / 102 / Synthetic glucocorticoid dexamethasone (DEX) is frequently used as a therapeutic agent to lessen the morbidity of chronic lung disease in premature infants. Pervious studies revealed that the neonatal DEX treatment would alter hippocampal synaptic plasticity in juvenile. Little is known about the long-term effect of neonatal DEX treatment on amygdale function. The current study is aimed to evaluate the long-term effect of neonatal DEX treatment on amygdale synaptic plasticity. To achieve this goal, male adult Wistar rats were subjected to receive subcutaneous injection (sc) of tapering doses of DEX (0.5 mg/kg, 0.3 mg/kg and 0.1mg/kg) from postnatal day 1 to 3, PN1~PN3. Animals were then subjected to electrophysiological recording at the age of 6 weeks. Our results showed that neonatal DEX treatment temporally decreased body weight of young rats, but did not affect that persistently to older age when compared with the control rats. In addition, neonatal DEX also treatment affected the formation of hippocampal long-term potentiation LTP. Neonatal DEX treated animals showed an apparent impairment on hippocampus LTP formation which can be partial restored by NMDA receptor agonist D-cycloserine. These results suggested that neonatal DEX treatment would exhibit a long-term adverse effect on hippocampal function. Further experiments will be required to elucidate the detail mechanism regarding to the long-term adverse effect of neonatal DEX treatment on hippocampal function.
| Identifer | oai:union.ndltd.org:TW/102NTNU5112039 |
| Date | January 2014 |
| Creators | Shi-Ping Peng, 彭詩屏 |
| Contributors | Kwok-Tung Lu, 呂國棟 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 75 |
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