碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 102 / Weight and length of tiger shrimp P. monodon postlarvae after 63 days of culture increased from 1.52 mg to 840 mg and from 0.88cm to 5.21 cm, respectively. Tiger shrimp juveniles (0.84±0.04g) reared at four different salinity levels of 5‰, 15‰, 25‰ and 35‰ after 20 weeks were examined for growth, activation of immunity, and immune response. Shrimp grew better at 25‰ and 35‰, and grew slower at 5‰. An in vitro study indicated that the phenoloxidase (PO) activity and the superoxide anion of shrimp hemocytes incubated in LPS and β-glucan at 0.1 mg ml-1 were significantly higher than those in control, and indicated these values were significantly higher in shrimp that reared at 25‰ and 35‰ than those of shrimp reared at 5‰. The immune parameters of shrimp reared at 5‰ were significantly lower than those of shrimp reared at 25‰ and 35‰. The hyaline cell (HC), granular cell (GC), PO activity and lysozyme activity of shrimp reared at 25‰ and 35‰ were significantly higher than those of shrimp reared at 5‰. Hemolymph protein and SOD activity of shrimp reared at 35‰ were significantly higher than those of shrimp reared at 5‰, 15‰, and 25‰. No significant difference in RB was found among four salinity levels. Phagocytic rate, phagocytic index, and clearance efficiency of shrimp reared at 25‰ and 35‰ was significantly higher than those of shrimp that reared at 5‰ and 15‰ when challenged with Vibrio alginolyticus. It was concluded that tiger shrimp P. monodon reared at 25‰ performed better in growth, activation of innate immunity in response to LPS and β-glucan, and higher immune parameters as well as resistance against Vibrio pathogen.
| Identifer | oai:union.ndltd.org:TW/102NTOU5086006 |
| Date | January 2014 |
| Creators | Vo Thi Tuyet Minh, 武氏雪明 |
| Contributors | Chen, Jiann-Chu, 陳建初 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 89 |
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