碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 102 / This study was aimed to determine the dietary effects of various antioxidant supplements on growth, survival, antioxidant capacity, immune response and oxidative stress resistance of Pacific white leg shrimp Litopenaeus vannamei. The seven supplements were astaxanthin (AX), vitamin A (VA), extract of Quillaja saponaria (QS), Yucca schidigera (YS), Nutrafito plus (NP, mix of QS and YS), leaves or seeds of Moringa oliefera (ML, MS). Each supplement was incorporated into a basal diet with the same diphenylpicrylhydrazyl (DPPH) antioxidant capacity. These 7 treatment diets and a control diet (C) without supplement were fed to the shrimp in triplicates for 12 weeks. Shrimp’s growth, survival, antioxidant capacity, immune response and hypoxia stress resistance were evaluated. The treatments had no effect on survival. Shrimps fed with supplements had better growth performance than C fed shrimps. As compared to the control, the treatment diets resulted in higher antioxidant capacity, namely, superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and immune response in respiratory burst (RB). AX shrimp had the highest and C shrimp the lowest antioxidant capacity in SOD, GPx and GR and resistance against hypoxia stress. Hypoxia stress increased shrimp’s GPx and AST, but had no effects on SOD and ALT. The treatments exhibited their effect on RB when under no stress, however, hypoxia stress overrode the treatment effect, causing no difference in RB and total haemocyte count (THC). Hypoxia stress increased glucose (Gluc) and lactate (Lac) and decreased triglycerides (Trigs). When under stress, treatments with supplements resulted in lower Gluc, Trigs and Lac and lethal dissolved oxygen level and longer lethal time than the control. However, there were no differences in those metabolic responses among treatments of the supplement. In conclusion, those antioxidants and plant extracts enhanced shrimp’s growth, antioxidant capacity and hypoxia stress resistance and stabilized their metabolic responses when under stress, however, only slightly affected their immune responses.
| Identifer | oai:union.ndltd.org:TW/102NTOU5086007 |
| Date | January 2014 |
| Creators | LAILA M. GALLEGO, 高萊菈 |
| Contributors | Chien,Yew-Hu, 陳瑤湖 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 75 |
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