碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 102 / This study used fermentor-batch culture Bacillus subtilis, Micrococcus luteus, Rhodobacter capsulatus and the commercial product -- Brobacking, which were supplemented into closed culture systems of white shrimp in different concentrations to investigate their effects on ammonia-N and nitrite-N elimination, pathogenic bacteria inhibition and white shrimp growth.
In the experiement of the effect of different probiotics on ammonia-N and nitrite-N concentrations in water, the results indicated that each treatment group reduced ammonia-N and nitrite-N concentration significantly compared to the control group (p＜0.05), while complex bacteria had better effect than single-species bacteria. In the experiment of their effect on vibrio inhibition, each treatment group could inhibit total vibrio counts to levels between 8.80#westeur024#102 cfu/ ml - 9.6#westeur024#102 cfu/ ml, which were significantly lower than the control group (p＜0.05). In the experiment of their effect on white shrimp growth, the highest growth rate (25.03%) appeared in group of Bacillus subtilis and Rhodobacter capsulatus complex bacteria; the best FCR and SGR (3.06 and 0.80) appeared in group of Micrococcus luteus and Rhodobacter capsulates complex bacteria; the best survival rate (88.89%) appeared in group of Bacillus subtilis and Rhodobacter capsulatus complex bacteria.
In the experiement of the effect of different concentrations of probiotics on ammonia-N and nitrite-N concentrations in water, the results showed that each treatment groups reduced ammonia-N and nitrite-N concentrations in water significantly compared to the control group (p＜0.05), while treatment with Bacillus subtilis and Rhodobacter capsulatus complex bacteria had better effect than treatment with Micrococcus luteus and Rhodobacter capsulates complex bacteria; also, 5 ppm had better results. In the experiment of their effect on vibrio inhibition, every treatment group, except for the one with 3 ppm of Micrococcus luteus and Rhodobacter capsulates complex bacteria, inhibited total vibrio counts to levels between 6.67#westeur024#102 cfu/ ml - 8.67#westeur024#102 cfu/ ml, which were significantly lower than the control group (p＜0.05). In the experiment of their effect on white shrimp growth, the highest growth rate (26.26%) appeared in group of 5 ppm of Bacillus subtilis and Rhodobacter capsulatus complex bacteria; the best FCR and SGR (2.98 and 0.78) appeared in group of 5 ppm of Bacillus subtilis and Rhodobacter capsulates complex bacteria; the best survival rate (92.59%) appeared in group of 5 ppm of Bacillus subtilis and Rhodobacter capsulatus complex bacteria.
In the experiement of the effect of commercial complex bacterias and self-made probiotics on ammonia-N and nitrite-N concentrations in water, the results revealed that each treatment groups reduced ammonia-N and nitrite-N concentrations in water significantly compared to the control group (p＜0.05), while treatment with Bacillus subtilis and Rhodobacter capsulatus complex bacteria had significantly better effect than treatment with Brobacking (p＜0.05) in early stage; however, the later had better effect than the former after 16 days. In nitrite-N, treatment with Bacillus subtilis and Rhodobacter capsulatus complex bacteria had better effect than treatment with Brobacking. In the experiment of their effect on vibrio inhibition, every treatment group inhibited total vibrio counts to levels between 7.93#westeur024#102 cfu/ ml-8.47#westeur024#102 cfu/ ml, which were significantly lower than the control group (p＜0.05). In the experiment of their effect on white shrimp growth, the highest growth rate (31.88%) appeared in group of Brobacking; the best FCR and SGR (2.58 and 0.92) appeared in group of Brobacking; the best survival rate (94.44%) appeared in group of Brobacking and group of Bacillus subtilis and Rhodobacter capsulatus complex bacteria.
Keywords: Probiotic, Water quality, Bacillus subtilis, Rhodobacter capsulatus, Micrococcus luteus
| Identifer | oai:union.ndltd.org:TW/102NTOU5086049 |
| Date | January 2014 |
| Creators | Chu, Yu-Ting, 朱鈺婷 |
| Contributors | Nan, Fan Hua, 冉繁華 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 51 |
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