碩士 / 國立臺灣海洋大學 / 食品科學系 / 102 / Carangidae species are edible coastal fish with importantly economic value, and the commercial products of which are popular in Taiwan. It is difficult to identify fish species from commercial fish products when the morphological characteristics are partially or completely lost after processing. Since the liver of Carangidae is considered as valuable supplements in diet habits of Taiwanese, the occurrence of food poisoning caused by ingesting the liver of large-sized fish is occasionally happened in Taiwan. In order to protect and prevent consumers from food intoxication in above-mentioned cases, a rapid method has been developed to identify 15 common Carangidae species and their commercial products in this study. In addition, the risk of ingesting liver of large-sized Caranx ignobilis (CI), Seriola dumerili and S. quinqueradiata has been assessed. First, polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) and DNA sequencing were used to establish RFLP map of 15 common Carangidae species in Taiwan. Second, the level of vitamin A in liver was examined by high performance liquid chromatography (HPLC), and the components of ciguateric toxins in liver was detected by mouse neuroblastoma cells Neuro-2a model and liquid chromatography - tandem mass spectrometry (LC-MS/MS). The developed PCR-RFLP method could be rapidly applied to identify the species of Carangidae both in fresh and commercial products in Taiwan. There is one fake product among in 10 commercial Carangidae products. The dose per unit of vitamin A has a positive correlation with the liver size and body weight of Carangidae fish. The vitamin A level in the liver of large-sized Carangidae is significant higher than the common fish. The existence of ciguatoxin (CTX) in the liver of sample CI-02 was detected by using developed cell test and LC-MS-MS, as approximately 0.08 ppb pacific-ciguatoxin-1 (P-CTX-1). In summary, the developed PCR-RFLP method in this study could be applied to distinguish authentication or fraudulent substitution of 15 Carangidae species, and used to identify causative residues that implicated in Carangidae fish-related food poisoning. The results indicate that large-sized Carangidae fish has a high level of vitamin A in the liver. This is the first research to demonstrate that the liver of large-sized Carangidae fish may accumulate P-CTX-1 via food chain in Taiwan. Acute vitamin A poisoning and ciguatera poisoning may occur simultaneously due to ingestion the liver of large-sized Carangidae.
| Identifer | oai:union.ndltd.org:TW/102NTOU5253020 |
| Date | January 2014 |
| Creators | Tseng, Yu-Hao, 曾玉豪 |
| Contributors | Hwang, Deng-Fwu, 黃登福 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 92 |
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