碩士 / 國立臺灣海洋大學 / 食品科學系 / 102 / Fish contain abundant nutrition and provide a good source of proteins. Fish are low-calorie, low-carbohydrate and relatively easy to digest, thus considered as a popular delicacy in many countries. While consumption of fish has been widely recommended, the health hazards related to heavy metal accumulation in fish have received public attention. In order to investigate the status of residual heavy metals in fish, 25 fish samples were purchased from traditional markets, supermarkets, warehouse stores and fish markets in Keelung from September to December in 2013, and analyzed by the official methods, “Test Method for Heavy Metals in Aquatic Animals-Test of Lead and Cadmium” and “General Method of Test for Heavy Metals” announced by the Ministry of Health and Welfare, Executive Yuan. Samples were digested with acid and determined by inductively coupled plasma mass spectrometer (ICP-MS). Concentrations of Pb, Cd, As, Cr, Ni, Se, Hg, Fe, Cu, Zn in the muscles and viscera were analyzed. Calibration curves of Pb, Cd, As, Cr, Ni, Se were linear in the range from 1 ～ 25 ng/mL, calibration curve of Hg was linear in the range from 0.2 ～ 5 ng/mL and calibration curves of Fe, Cu, Zn were linear in the range from 10 ～ 1,000 ng/mL. The correlation coefficients for all the calibration curves were at least 0.9996, showing good linear relationship. The accuracy of the analytical procedures was verified by analysis of certificated reference material (DORM-4). The results were in agreement with certified values. Recoveries were ranged from 80.4% ～ 117.8%, showing good accuracy. The mean levels found in fish muscles were as follows: As, 1.275 mg/kg (0.070 ～ 5.027 mg/kg); Cd, 0.015 mg/kg (0.001 ～ 0.139 mg/kg); Cu, 0.315 mg/kg (＜0.01 ～ 1.300 mg/kg); Pb, 0.004 mg/kg (＜0.001 ～ 0.032 mg/kg); Hg, 0.024 mg/kg (＜0.001 ～ 0.088 mg/kg); Ni, 0.011 mg/kg (＜0.001 ～ 0.088 mg/kg); Zn, 8.41 mg/kg (3.60 ～ 30.69 mg/kg); Cr, 0.044 mg/kg (＜0.001 ～ 0.152 mg/kg); Se, 0.601 mg/kg (0.205 ～ 2.347 mg/kg); Fe, 7.96 mg/kg (1.63 ～ 24.83 mg/kg). In general, heavy metal concentrations of fish in this study were lower than other reports, indicating that heavy metal pollution was minor in this investigation. The fish samples have shown to be compliant with the hygiene standards set by the Ministry of Health and Welfare, Executive Yuan, showing that adverse health effects were absent.
Keywords：fish samples, ICP-MS, certificated reference material, heavy metals.
| Identifer | oai:union.ndltd.org:TW/102NTOU5253034 |
| Date | January 2013 |
| Creators | Fu, Shu-Ying, 傅淑英 |
| Contributors | Hwang, Deng-Fwu, 黃登福 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 68 |
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