碩士 / 國立臺灣海洋大學 / 食品科學系 / 102 / Traditional cancer therapies are high efficiency to kill cancer cells, but some of the advanced malignancy cells can still survive. Recent studies have shown that the small population of tumor cells, known as cancer stem cells (CSCs). These CSCs have the ability to promote tumor drug-resistant and metastasis, and play an important role of malignant progression and recurrence. Colorectal cancer (CRC) is one of the common malignant neoplasm, that is difficult to discover in the early stage and often metastasizes to liver and lung. Omega-3 polyunsaturated fatty acids (PUFAs) derived from fish oil has been reported has the capability to inhibit tumor growth and metastasis. Selenium yeast is also able to suppress tumor invasion and anti-angiogenesis. However, whether the combination effects of fish oil and selenium yeast mitigate the adverse effects of CSCs is still unclear. First, we treat cells with chemotherapy drug (cisplatin) to characterize the drug resistant of CSCs in BALB/c mouse colon carcinoma cells (CT26). The results showed that the survival cells have higher chemo-resistance and CSCs-related gene expression. Therefore, we establish CT26 as a colon CSCs research model in the following experiments. We screen the side population cells (SP cells) in CT26 with Hoechst dye, which have better drug- resistance and higher CSCs-related gene expression. Furthermore, the migratory capacity and colony formation ability of SP cells are higher than non-population cells (NSP cells). In vivo, we demonstrate that SP cells have stronger tumorigenic ability, more body weight loss and lung metastasis. After analyses lung tissues, the gene expression of metastasis–associated chemokines and matrix metalloproteinases are increased. In further experiments, the dietary supplementation with fish oil and selenium yeast can attenuate body weight loss and lung metastasis. The expression of metastasis–associated chemokines and matrix metalloproteinases are suppressed. Thus, fish oil and selenium yeast supplementation can lower the possibility of cancer metastasis which caused by cancer stem cells.
| Identifer | oai:union.ndltd.org:TW/102NTOU5253051 |
| Date | January 2014 |
| Creators | Yu, Wen-ya, 余雯雅 |
| Contributors | Wu, Chang-Jer, 吳彰哲 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 74 |
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