碩士 / 國立臺灣海洋大學 / 食品科學系 / 102 / The aim of this study is to produce lactic acid from three kinds of algal polysaccharide hydrolysates (Gracilaria sp., Sargassum siliquosum, and Ulva lactuca), which sequentially hydrolysis by hot acid, commercial enzymes, and crude enzymes from Pseudomonas vesicularis MA103 (MA103) and Aeromonas salmonicida MAEF108 (MAEF108) and fermented by lactic acid bacteria (LAB). Reducing sugar content per kWh electricity usage of 10% (w/v) Gracilaria sp., S. siliquosum, and U. lactuca powder hydrolyzed by 0.4 N HCl at 121oC for 30 min were 19.76, 11.07, and 15.99 mg/mL, respectively. MA103 incubated in artificial sea water medium added with hot water extracted Gracilaria sp. polysaccharide and HCl-extracted Gracilaria sp. polysaccharide (AM-Gra-AP) enriched with defatted soybean flour for 2 d, amylase activity was 6.76 U. While MAEF108 incubated in AM-Gra-AP that enriched with yeast extract for 3 d, agarase activity was 2.76 U. MA 103 and MAEF108 incubated in artificial sea water medium added with hot water extracted S. siliquosum polysaccharide (AM-Sar-P) that enriched with defatted soybean flour for 2 d, alginate lyase activity were 0.61 and 0.37 U, respectively. In artificial sea water medium added with hot water extracted U. lactuca polysaccharide and HCl-extracted U. lactuca polysaccharide (AM-Ulv-AP) that enriched with defatted soybean flour for 2 day, MA103 showed 5.30 U amylase activity and MAEF108 showed 0.26 U agarase activity. LAB selection showed that strains BCRC 10695, 12327, and isolate KP5 fermented in Gracilaria sp. polysaccharide hydrolysate resulted in lactic acid concentration of 6.96, 6.31, and 4.54 g/L, respectively. Strains BCRC 10695, 12327, and 14068 fermented in S. siliquosum polysaccharide hydrolysate resulted in lactic acid concentration of 5.38, 5.54, and 5.17 g/L, respectively. While strains BCRC 10695, 12327, and 14068 fermented in U. lactuca polysaccharide hydrolysate resulted in lactic acid concentration of 7.49, 8.37, and 7.96, respectively. Gracilaria sp. polysaccharide hydrolysate prepared by procedure A, 10% (w/v) seaweed power hydrolyzed by 0.4N HCl at 121oC for 30 min, 7,600 U cellulase, crude enzymes from MA 103 and MAEF 108 incubated in MMB-Gra and fermented by combination of 3% (v/v) Lb. acidophilus BCRC 10695 and 3% (v/v) Lb. plantarum BCRC 12327 with 0.5% (w/v) yeast extract as nitrogen sources resulted in 19.32 g/100 g lactic acid yield. Procedure B, conditions were the same as procedure A, except MMB-GraMA103 and MMB-GraMAEF108 were replaced by AM-Gra-APMA103 and AM-Gra-APMAEF108. In this hydrolysate, fermented by combination of 3% (v/v) Lb. acidophilus BCRC 10695 and 3% (v/v) Lb. plantarum BCRC 12327 with 0.5% (w/v) yeast extract as nitrogen sources resulted in 20.58 g/100 g lactic acid yield. Procedure C, with 5% (w/v) biomass loading extracted by 0.4N HCl at 121oC for 20 min and higher level of commercial enzyme compare to procedure A and used MMB as crude enzymes inducing medium, fermented by combination of 3% (v/v) Lb. acidophilus BCRC 10695 and 3% (v/v) Lb. plantarum BCRC 12327 with 0.5% (w/v) yeast extract as nitrogen sources resulted in 49.10 g/100 g lactic acid yield. Procedure A, B, and C of S. siliquosum polysaccharide hydrolysate were added 0.5% (w/v) okara as nitrogen sources fermented by combination of 3% (v/v) Lb. acidophilus BCRC 10695 and 3% (v/v) Lb. plantarum BCRC 12327 at 30oC, the lactic acid yield were 14.51, 14.58, 59.16 g per 100 g dried S. siliquosum, respectively. Procedure A, B, and C of U. lactuca polysaccharide hydrolysate were added with 5% (w/v) yeast extract as nitrogen sources fermented by LAB strain 6% (v/v) Lb. plantarum BCRC 12327 at 30oC, the lactic acid concentration were 12.19, 12.89, 50.22 g per 100 g dried U. lactuca, respectively.
| Identifer | oai:union.ndltd.org:TW/102NTOU5253064 |
| Date | January 2014 |
| Creators | Kao, Te-Yu, 高德育 |
| Contributors | Pan, Chorng-Liang, 潘崇良 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 143 |
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