碩士 / 國立臺灣海洋大學 / 食品科學系 / 102 / The aim of this study is to investigate the optimal production condition of alginate oligosaccharides from Sargassum sp. And its effect on biological activities including antioxidant, anticoagulant, antivirus, prebiotic potential, and the power of inhibit angiotensin converting enzyme (ACE). With the usage of different concentration of Na2CO3 to extract alginate from Sargassum sp. It is found that when the concentration is 5.0%, the yield of alginate is 25.29%, with the increasing of extraction time from 1 to 4 hr, the yield of alginate is higher and can reach to 26.35% when the extraction time is 4 hr. This study also discusses about the changes of reducing sugar and total sugar contents of 0.5% alginate solution under different hot and acid hydrolysis conditions, for the purpose of finding the optimal hot and acid hydrolysis condition of alginate solution. The results shows that procedure G can make the contents of reducing sugar up to 0.31 ± 0.01 mg/mL and total sugar contents can reach 1.01 ± 0.03 mg/mL, and this study uses procedure G as the optimal hot and acid hydrolysis condition of 0.5% alginate solution to go on the following procedure of hydrolysis. After treated with procedure G, 0.5% alginate solution was hydrolysis with the alginate lyase rpalIII crude eneyme induced by nisin from Lactococcus lactis NZ3900-rpalIII-14 with 48 hr, and distinguishing the oligosaccharides whose molecular weight lower than 3 kDa with ultrafiltration (UF), finally, separate with the gel permeation chromartography (GPC). This procedure can obtain a single oligosaccharide peak. Collected this peak and lyophilizated it to obtain powder, and analyzing with high performance liquid chromatograph (HPLC) can know that the retention time is 28.02 min. With the comparison of the calibration curve of different degree of polymerization (DP) molecular mass maltoolgosaccharide, it is distinguished as the DP of this oligosaccharide is 2, and is named as Alg-S2. The molecular weight is also analyzed with thin layer chromatography (TLC) and the Rf value is 0.477, and the spectrum of fourier transform infrared spectroscopy (FT-IR) of Alg-S2 indicates that it has the signal of hydroxyl group (O-H), pyranose, sulfate group, and carboxyl group. Calculate the yield of Alg-S2 is 15.04 g/100 g Sargassum powder, the content of sulfate is 0.11%, and the content of total phenol was not detected. The DPPH radical scavenging effect of alginate oligosaccharides (< 3 kDa) and Alg-S2 can reach 31.25% and 25.03%, respectively, equal to the effect of 37.09 and 29.71 μg/mL trolox, the reducing power equal to 10.68 and 6.94 μg/mL trolox, and the ferrous ion chelating effect are 11.58% and 21.95%, respectively. Alginate oligosaccharides (< 3 kDa) and Alg-S2 can delay the ctivated partial thromboplastin time (APTT) to 12-60 sec and can decrease the aggregation level when the concentration are 5 mg/mL or 20 mg/mL. It can also delay the thromboplastin time (TT) to 12-60 sec, and lowering the aggregation level. Alginate oligosaccharides (< 3 kDa) and Alg-S2 do not have the effect of delaying prothrombin time (PT), but Alg-S2 can descend the aggregation level. Alginate oligosaccharides (< 3 kDa) and Alg-S2 can increasing the cell viability of BHK-21 cells infected by Japanese encephalitis virus on pre-treated group from 69.27% to 94.76% and 100.53% when the concentration is 100 μg/mL, and Alg-S2 can increasing the cell viability of BHK-21 cells infected by Japanese encephalitis virus from 70.02% to 95.70% on co-treated group in the same dosage, but have no significant effect on other groups. It has no significant effect on viable count and pH value to Lb. acidophilus, Lb. plantarum, Bifid. adolescentis, Bifid. bifidum, and Bacillus licheniformis FRI MY-55 in 0.5% yeast extract broth when the concentration of alginate oligosaccharides (< 3 kDa) and Alg-S2 is 2 mg/mL. When the concentration is 1 mg/mL or 2 mg/mL, alginate oligosaccharides (< 3 kDa) can inhibit 4.50% and 7.07% ACE activity, but Alg-S2 has no effect to inhibit ACE activity.
| Identifer | oai:union.ndltd.org:TW/102NTOU5253069 |
| Date | January 2014 |
| Creators | Wei, Ming-Yi, 魏銘毅 |
| Contributors | Pan, Chorng-Liang, 潘崇良 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 82 |
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