碩士 / 國立臺灣大學 / 生化科技學系 / 102 / Probiotics is defined as the microbe that beneficially affect the host. By influencing the intestinal macrophage and dendritic cell, probiotics may stimulate the host immune response or promote the immune-modulation function. Fish oil alleviates the excessive and chronic inflammation by reducing the phagocytic activity of macrophage and pro-inflammatory cytokine level. Therefore, we wondered that whether the combination of probiotics and nutrition supplement containing fish oil would multiple the effects of immune-modulatory function.
In this study, the mixture of Lactobacilus rhamnosus and Bifidobacterium lactis were used as the source of probiotics and the nutrition supplement contains fish oil and various micronutrients. There were two parts in this experiment: non-specific and specific immune response. In the first part, nine-week-old BALB/c female mice were tube-fed with different contents including probiotics alone, nutrition supplement alone or combined sample for 6 weeks and followed by performing sacrifice to measure the innate and non-specific adaptive immune indicators. The results showed that combined-sample group significantly increased activity of NK cell, elevated total IgM, IgG, IgA in the serum and reduced IL-2 secretion of splenocytes. Knowing that the immune-modulatory ability of combined-sample group, we continued with the next part. In the experiment, nine-week-old BALB/c female mice were not only fed by different sample for 9 weeks but also immunized with OVA, followed by sacrifice. The innate immune responses and adaptive immune responses including OVA-specific and non-specific were examined. The results showed that combined-sample group significantly induced B cell proliferation, elevated total IgG, IgA in the serum, and significantly increased IL-10 but reduced IL-2 secretion of splenocytes. Combined-sample group also tend to induce phagocytic activity and T cell proliferation. In the OVA-specific indicators, combined-sample group tend to increase OVA-specific IgG and significantly increased IL-2 secretion with OVA- stimulated splenocytes.
In conclusion, combined-sample group is the most remarkable to modulate the immune system within other single-sample groups, especially the elevated level of total antibodies. Supporting that supplied BALB/c mice with probiotics combined nutrition supplement containing fish oil may benefit the immune system than the single sample group, which may avoid the extracellular pathogen invasion and promote mucosal immunity.
| Identifer | oai:union.ndltd.org:TW/102NTU05106007 |
| Date | January 2014 |
| Creators | Ya-Ching Chang, 詹雅晴 |
| Contributors | 林璧鳳 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 108 |
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