博士 / 國立臺灣大學 / 海洋研究所 / 102 / Various extracts of the marine brown alga Endarachne binghamiae were assayed
in vitro for inhibiting the proliferation in human breast adenocarcinoma cell
（MDA-MB-231）, human hepatoblastoma cell（Hep G2）and human pancreatic cell
（MIA PaCa-2）. In the present study, fucoidan, alginic acid, calcium alginate and
sodium alginate extracted from the brown alga Petalonia binghamiae inhibited
markedly the proliferation in carcinoma cells. The inhibitory reaction of sodium
alginate decreased after being treated with citrate buffer solution and lost in the
presence of Ca2+, Zn2+, Co2+ or Mn2+. However, sodium alginate remained high
inhibitory activity if metal ions were treated with Na2EDTA prior to addition to
culture medium. These results showed that ion exchange at carboxyl groups as well as
molecule conformation of the polymer played an important role in alginate inhibitory
reaction with carcinoma cells. Moreover, the obtained sodium alginate induced
murine macrophages to produce proteinaeous substance that significantly inhibited
carcinoma cell proliferation. My study suggests that sodium alginate has using
potential in anticancer therapy and research due to its wide distribution and high
production in marine brown algae. A novel protein assigned as EBP (Endarachne
binghamiae protein) was obtained after being extracted in phosphate buffered saline,
precipitated by saturated ammonium sulfate and finally purified by gel filtration and
ion-exchange column chromatography. The purified protein has molecular weight of
69 kDa. It is a monomer glycoprotein, containing 20.37 % carbohydrate moiety. The
EBP is rather stable in immunity while subjected to 121℃ and a wide range of pH
values (3.0~12.0). The above findings suggests that the reported alga is a promising
source of proteins and polysaccharides. In detail research, we are looking forward to
seeing that immunostimulation and immuno-modulation can be used as medicine in
cancer therapy.
| Identifer | oai:union.ndltd.org:TW/102NTU05279034 |
| Date | January 2014 |
| Creators | Hui-Ting Lee, 李惠婷 |
| Contributors | , 黃穰, 孫志陸 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 126 |
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