The effect of β-glucan on innate immune responses of Litopenaeus vannamei and its resistance against Vibrio alginolyticus infection / 菇蕈多醣體對白蝦先天性免疫能力的影響以及抵抗溶藻弧菌的能力

碩士 / 國立臺灣大學 / 漁業科學研究所 / 102 / The purpose of this study is using feed with different concentrations of β-glucan on white shrimp, and examine the variance of total haemocyte count (THC), prophenoloxidase (proPO), superoxide anion (O2-), phagocytic rate (PR), phagocytic index (PI). It is investigate the effect of β-glucan on innate immune response of white shrimp and observed the immune parameter, survival rate of white shrimp which injected the pathogens at different fed time points. It can be analyze the appropriate dosage and frequency of feeding for the aquaculture industry.
Section I, different dietary doses (1、2、4 g / kg diet) of β-glucan were used. The result showed that the THC, proPO, O2- production ratio, PR and PI were higher significantly (P<0.05) than the control group. The high-dose group (4 g / kg diet) can enhance the immune parameters fastest (THC, proPO, PR, PI). The low-dose group (1 g / kg diet) take a more time to increase immune parameters.
Section II, white shrimp after injected the Vibrio alginolyticus compared with the Blank group, the immune parameters were significantly reduced. The high-dose group (4 g / kg diet) can enhance the immune parameters fastest, and the survival rate of white shrimp which injected V. alginolyticus after 48h at different fed time points is 40、80、70、60、70、80 % (day 0、1、3、5、7、10). The low-dose group (1 g / kg diet) take a more time to increase immune parameters, and the survival rate of white shrimp which injected Vibrio alginolyticus after 48h at different fed time points is 40、70、50、50、60、60 % (day 0、1、3、5、7、10).
These results indicate β-glucan can effectively enhance the innate immune response and the resistance against Vibrio alginolyticus of white shrimp.

Identiferoai:union.ndltd.org:TW/102NTU05451012
Date January 2014
CreatorsMin-Feng Yang, 楊珉楓
ContributorsShiu-Nan Chen, 陳秀男
Source SetsNational Digital Library of Theses and Dissertations in Taiwan
Languagezh-TW
Detected LanguageEnglish
Type學位論文 ; thesis
Format67

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