碩士 / 國立臺灣大學 / 生命科學系 / 102 / D-loop (mitochondrial/mtDNA) and microsatellites (nuclear) could assign individuals to populations for aquaculture, test marine biogeographical hypotheses on the distribution of organisms and identify populations to be prioritized for conservation. The black tiger shrimp, Penaeus monodon, is ideal for d-loop and microsatellite analyses because it is widely distributed throughout a biogeographic line called Huxley’s Line and monitoring of wild populations is necessary to maintain the quality of the shrimp seed sources.
This study amplified d-loop sequences and tested six microsatellites on wild populations of black tiger shrimp from Leyte; Surigao del Norte and Pangandaran, West Java. Thirty individuals were collected from each population. The proportions of properly assigned samples using microsatellites were 100% in Pangandaran and Surigao and 92.31% in Leyte. No population was considered as an evolutionary significant unit because there was no reciprocal monophyly based on the resulting phylogenetic tree. Only Leyte combined with Surigao can be considered as management unit because it passed a test on significant divergence of allele frequencies at nuclear loci. Pleistocene sea levels and present-day ocean circulation rather than the Huxley’s line alone affect the distribution of the three populations
| Identifer | oai:union.ndltd.org:TW/102NTU05525021 |
| Date | January 2014 |
| Creators | Claire Samantha T. Juanico, 柯蕾 |
| Contributors | Alex Hon-Tsen Yu, 于宏燦 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 91 |
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