碩士 / 國立高雄大學 / 化學工程及材料工程學系碩士班 / 102 / This study combines molecular imprinting technology, chitosan and magnetic nanoparticles to prepare magnetic nucleobases imprinted chitosan composite nanoparticles (MNIC-NPs) as gene vectors. During the experiments, we imprint adenine, cytosine and thymine on chitosan and use them for gene transfection and induction of cancer cells apoptosis. Using phase transformation to prepare MNIC-NPs in aqueous solution can maintain the nature properties of chitosan and simplify the system, which also can decrease cost and prevent excess cytotoxicity. Imprinting 0.1g/L nucleobases on 0.01wt% chitosan with 370k M.W., then analyzing properties by high performance liquid chromatography, transmission electron microscopy, particle size analyzer and Raman spectrum. Chitosan can reduce 35~50% cytotoxicity of magnetic particles which dose to HEK293 and HepG2 cells. Adsorb green fluorescent protein gene (pEGFP) with cytosine imprinted polymers (CIPs) and transfect into HEK293 cells by magnetic field, the efficiency can surpass 50% which compare with commercial vectors (Lipofection 2000). After dosing thymine imprinted polymers (TIPs, which are expected to block the function of telomeres) into HepG2 cells, the growth rate slowing down obviously. Furthermore, rebinding artificially short telomeres on TIPs before dosing can successfully induce apoptosis of HepG2.
| Identifer | oai:union.ndltd.org:TW/102NUK05063069 |
| Date | January 2014 |
| Creators | Jian-Zhou Chen, 陳建州 |
| Contributors | Hung-Yin Lin, 林宏殷 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 115 |
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