碩士 / 國立高雄大學 / 生命科學系碩士班 / 102 / A clonal cell line (ARB8-2) isolated from the brain of cichlid fish (Aequidens rivulatus) was investigated by reverse transcription polymerase chain reaction (RT-PCR),Western blot, immunocytochemistry, PCR and Real-time PCR were utilized to characterize the molecular expression of ARB8-2 cell line. The results showed that the ARB8-2 express radial glia markers such as nestin and brain lipid binding protein (BLBP), astrocyte markers such as glial fibrillary acidic protein (GFAP), glutamine synthetase (GS) and vimentin, oligodendrocyte progenitor cell marker such as A2B5, neuron markers such as tyrosine hydroxylase (TH), epithelial cell markers such as gap junction connexin 43 (Cx43) and tight junction occludin, melanocyte markers such as tyrosinase, tyrosinase-related protein-2 (TRP-2), microphthalmia-associated transcription factor (MITF), SLC45A2 and keratinocyte-associated transmembrane protein 2 (KCT-2) and retinal epithelial marker retinal pigment epithelium-specific 65 (RPE65). However, the ARB8-2 also express homeodomain-containing transcription factors NKX 2.3 and NKX 6.2 and GATA 3. The results indicated that ARB8-2 might be the neural stem cell.
| Identifer | oai:union.ndltd.org:TW/102NUK05105005 |
| Date | January 2014 |
| Creators | Shang-wei Yeh, 葉上暐 |
| Contributors | Chiu-Ming Wen, 溫秋明 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 105 |
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