碩士 / 國立臺北科技大學 / 化學工程研究所 / 102 / In this experiment, cell was mixed in 5% RGD-alginate and used rapid prototyping system to build the 3D porous scaffolds. The experimental group (E) was cyclically stretched for 1 min per day with a frequency of 15 rpm (E-15) and 60 rpm (E-60), respectively; the control group (C) was static culture. The cells were harvested on day 1, 4, 7, 14.
The cell proliferation results show that the cells embedded in alginate fibers grew overtime and that day 7 the cells subjected to stretching grew slightly faster compared to those under static culture. The viability of cells under static culture was higher than that of stretched cells in earlier days (day 1 and 4). As the culture time increased (day 7 and 14), the viability of stretched cells increased and surpassed that of static cells. We found that live/dead staining appeared the cells were mostly survival in day 1, 4, 7, 14. HE staining showed fibroblast stained cultures after 14 days of cyclic stretch have no apparent preferential orientation. Others have shown stretching of cells changes cell morphology. We did not observe this in our experiments. Immunofluorescence staining showed that type I collagen found around the cell and no collagen increased.
| Identifer | oai:union.ndltd.org:TW/102TIT05063075 |
| Date | January 2014 |
| Creators | Min-Che Wu, 吳旻哲 |
| Contributors | 林忻怡 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 51 |
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