碩士 / 臺北醫學大學 / 醫學檢驗暨生物技術學系所 / 102 / The problem of elderly care has become increasingly evident in developed countries in recent years due to their aging societies. One of the most important concerns of elderly care is the prevention and treatment of Alzheimer''s disease (AD). AD is a progressive neurodegenerative disease. Early symptoms are not obvious, and the diagnosis relies heavily on the clinical staff’s experience, such as using MMSE scores. There is no definitive diagnosis biomarker to date. Therefore we wanted to find specific proteins in patients’ serum to identify effective diagnostic biomarkers for AD and help track and treat the disease. Studies have shown that the neurodegenerative Huntington’s disease (HD)-associated protein (Hap1) was involved in the intracellular transport of AD-related amyloid precursor protein (APP).We checked the changes of these related proteins in AD patients’ serum and tried to develop a new diagnostic method without subjective bias. In the present study, we detected the concentrations of Abelson helper integration site-1 (AHI-1; Jouberin), dynein-1 light intermediate chain (DYNC1LI)-2 and kinesin light chain (KLC)-2 in the serum of healthy people and patients using enzyme-linked immunosorbent assay (ELISA). We found significant differences between the control healthy subjects and AD patients. Then we used support vector machine (SVM) to establish a model combining AHI-1, DYNC1LI2, KLC2, age, and sex to predict AD risks. Our results showed that the best AD biomarker in serum is DYNC1LI2. A combination of DYNC1LI2 with other markers showed a higher area under curve (AUC) in receiver operating characteristic (ROC) curve.
| Identifer | oai:union.ndltd.org:TW/102TMC05108015 |
| Date | January 2014 |
| Creators | Jian-Yu Chen, 陳建宇 |
| Contributors | 林詠峯 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 74 |
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