碩士 / 臺北醫學大學 / 生醫材料暨組織工程研究所 / 102 / Microcapsulation has been widely used in pharmaceutical, food, and biotechnology. In this study, we focused on design alginate bead formulations, encapsulating hydrophilic drugs - Rose Bengal（RB）and hydrophobic drugs –lutein/zeaxanthin. The effects of sodium alginate to polymer ratio, CaCl2 concentration and crosslinking time on the encapsulation efficiency, particle size, hardness, and spreadability of the beads were optimized by using response surface methodology（RSM）.
Our results showed the encapsulation efficiency of RB was significantly affected by the concentration of carboxymethyl cellulose （CMCH）, CaCl2 concentration as well as the crosslinking time. Size of the beads containing RB increased by increasing the content of CMCH. The hardness and spreadability of the beads increased by increasing the concentration of CMCH, CaCl2 and cross-linking time. On the other hand, the encapsulation efficiency, particle size, hardness and spreadability of alginate beads containing hydrophobic drug were strongly affected by the concentration of CMCH and CaCl2.
In addition, the low encapsulation efficiency of alginate beads containing RB was found. Thus, adding gelatin, PVP K40, and different preparation methods（gradient method、alginate capsule）were selected to improve its encapsulation efficiency. The results showed that adding gelatin（0.5%）to the formulation could increase the encapsulation efficiency from 67% to 98% without changing the texture and shape of the beads. Adding PVP K40 or modifying the preparation methods was found to enhance the encapsulation efficiency, but the beads became harder and were not easy to smear. Stability test of alginate beads showed that the beads can prevent photobleaching of RB from light, and the degradation rate of Lutein/zeaxanthin greatly affected by the temperature.
In conclusion, the formulation optimization of alginate beads using as topical drug delivery system can be obtained by adjusting sodium alginate to polymer ratio, CaCl2 concentration and crosslinking time. Moreover, sodium alginate to polymer ratio seems to play the most important role regarding size, encapsulation efficiency and hardness and spreadability of the alginate beads forming.
| Identifer | oai:union.ndltd.org:TW/102TMC05723001 |
| Date | January 2014 |
| Creators | Hsien-Ting Cheng, 鄭先廷 |
| Contributors | Tsuimin Tsai, 蔡翠敏 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 92 |
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