碩士 / 國立中正大學 / 化學工程研究所 / 103 / Curcumin and nerve growth factor (NGF) encapsulated into cardiolipin (CL) liposomes affeced phosphorylation of protein in this reserch. We used β-Amyloid (Aβ) to induce phophorylation of protein in SK-N-MC cells for simulating Alzheimer’s disease (AD). Our results suggest that Aβ induced level of phosphorylation is more than control groups and curcumin encapsulated into cardiolipin liposomes inhibit significantly phosphorylation of p38, JNK and S202. NGF encapsulated into cardiolipin liposomes can increase p-ERK5 expression significantly. Immunocytochemistry of p-p38 and p-JNK indicates apoptosis for treating SK-N-MC cells with 10 μM Aβ for 24 h. Futhermore, in an AD rat model by the hippocampus injection of Aβ, WGA-CL liposomes significantly decrease Aβ plaques area and level of lipid peroxidation. WGA-CRM-CL liposomes also can protect Nissl bodies against Aβ-induced degeneration. According to our results, we conclude that WGA-CL liposomes can be a useful delivery system for AD treatment in preclinical trials.
| Identifer | oai:union.ndltd.org:TW/103CCU00063065 |
| Date | January 2015 |
| Creators | Zhe-Yu Lin, 林哲宇 |
| Contributors | Yung-Chih Kuo, 郭勇志 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 89 |
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