碩士 / 大葉大學 / 生物產業科技學系 / 103 / White spot syndrome virus (WSSV, genus Whispovirus, family Nimaviridae) is a wide occurring virus which attacks cultured shrimp and many other crustaceans and has caused huge economic losses for the global shrimp farming industry. Until now effective methods for dealing with the disease caused by wssv infection is not available.shrimp cell laminin receptor (lamr) was reported as a wssv infection related protein receptor. Our previous studies found that the Penaeus monodon lamr (PmLamr) interacted with WSSV structural proteins, such as VP26, VP31 and VP187-C (the C-terminal portion of VP187). In this study by co-immunoprecipitation assays, interactions between PmLamr and VP31 and VP187-C were confirmed, and interaction with another protein, the VP187-N were also identified.one of these three PmLamr interacted protein, the VP31, was then subjected to further analysis. Membrane topology assay indicates that VP31 is an envelope protein with large portion expose outside of the WSSV virion. Results of this study comfirm the Lamr is an important receptor for WSSV’s infection and suggest WSSV envelope protein VP31 plays the major role in this virus-receptor interaction. Membrane topology assay indicates that VP31 is an envelope protein with large portion expose outside of the WSSV virion. Results of this study comfirm the Lamr is an important receptor for WSSV’s infection and suggest WSSV envelope protein VP31 plays the major role in this virus-receptor interaction.Enzyme-link immunosorbent assay showed that interaction between recombinant His-PmLamr (rHis-PmLamr) and recombant MBP-VP31 (rMBP-VP31) was with a dose dependent manner, and their interaction could be competed by the presence of wssv virion. In vivo WSSV challenge assay showed that VP31 treating effectively deoseasing the mortality of WSSV challenged shrimp, and such effect could be vanished by adding of the rPmLamr. Data from this study will provide valuable information in understanding of the wssv infection process, and also help in developing anti-WSSV agents.
| Identifer | oai:union.ndltd.org:TW/103DYU00111006 |
| Date | January 2015 |
| Creators | Wu Chun Ju, 吳純如 |
| Contributors | Li Shih Chieh, Chang Yun Shiang, 李世傑, 張雲祥 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 73 |
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