碩士 / 大葉大學 / 生物科技碩士在職學位學程 / 104 / Trehalose is a non-reducing disaccharide which widely present in various living organisms and be used as energy storage or a carbon source. When the organism is under extreme growth environment, such as high salt, high pH, high temperature, low temperature, or abnormal osmotic pressure etc., the cells will secrete more trehalose to protect themselves.
To investigate the effect of different reverse culture conditions on trehalose accumulation in the yeast cells. In this study, we plan to stimulate the secretion of trehalose in vivo using different reverse environment. Different kinds of stress factors were used as follow : pH (pH 6.0 ~ 9.0); temperature (20 ℃~40 ℃); incubation time (1 to 5 days); different nitrogen source and concentration: NH4NO3, peptone, yeast extract, NH4Cl, KNO3, NH4H2PO4, and (NH4)2SO4 (0.5 - 1.0%，w/v); various inorganic salts and concentration: MgCl2．6H2O, CaSO4, FeSO4．7H2O and MgS04．7H2O (0.5 -1%，w/v). After high-temperature extraction , the result shows that the addition of 1% KNO3, 1% MgCl2．6H2O in the medium, and incubated at 30 ℃ for 4 days, to effectively enhance 7.2 times of the trehalose accumulation in vivo. The optimized reverse fermentation conditions, for intracellular trehalose accumulation of Candida rugosa can be another new selection for further industrial applications in the future.
Key Words: Candida rugosa, Extraction, Fermentation, Stress, Trehalose
| Identifer | oai:union.ndltd.org:TW/103DYU01613006 |
| Date | January 2015 |
| Creators | Bao-Hua Huang, 黃寶華 |
| Contributors | Shu-Wei Chang, 張淑微 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 67 |
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