碩士 / 輔仁大學 / 生命科學系碩士班 / 103 / Title: Bioinspired chlorella vulgaris extracts/poly(vinyl alcohol) nanofibers by electrospinning for wound dressing.
Background: When the skin tissue trauma, would make a wound. According to the different time of wound healing, is divided into acute wounds and chronic wounds. Wound dressing can promote wound healing and reducing scarring. In the pass, traditional dressings, like cotton balls, gauze, bandages have been unable to handle the current diverse wound. Today, with the advances in biomedical technology, advanced wound dressing market is widely. According to the different types of product function can be divided into Moist wound dressing、silver dressing、biomaterials、negative pressure wound therapy (NPWT).
Aim: In this study, using high biocompatible polyvinyl alcohol (PVA) as polymer materials, and adding Chlorella vulgaris extracts (CVE) to prepare bioinspired CVE/PVA nanofibers with a large surface area and pore structure by elecrospinning. By its biomimic properties with nature extracellular matrix to evaluate potential as a wound dressing.
Materials and Methods: MTT assay to compare cell cytotoxicity and cell proliferation in CVE to HS-68. ECIS to observe cell migration and observing CVE antibacterial effect. Preparing CVE/PVA nanofibers by electrospinnning. Observing CVE/PVA nanofibers structure and HS-68 cell attachment. Using MTT assay and total collagen to cell Cytotoxicity and extracellular matrix proliferation. In animal experiment, using ICR mice, nanofiber attached on the ventral wound, observing the appearance of the wound and H & E staining of skin tissue at the wound patterns change.
Results: At an appropriate concentration, CVE can promote cell proliferation. ECIS result show CVE can promote cell proliferation and cell migration. By electrospining, CVE/PVA nanofiber can promote cell and extracellular matrix proliferation. In animal experiments, CVE/PVA nanofiber can promote wound healing. However CVE/PVA nanofiber and treat only CVE do not have antimicrobial activility.
Conclusion: Results prove that CVE/PVA nanofiber can promote wound healing. CVP-918 have the best result. The experimental result show CVP-918 have the potential as wound dressing.
Keywords: Chlorella vulgaris extracts, polyvinyl alcohol, electrospining , wound dressing, tissue repair.
| Identifer | oai:union.ndltd.org:TW/103FJU00105006 |
| Date | January 2015 |
| Creators | Ting-Yu,Li, 黎亭佑 |
| Contributors | Hsia-Wei,Liu, 劉席瑋 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 35 |
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