碩士 / 弘光科技大學 / 食品科技所 / 104 / Cordyceps militaris is an Entomopathogenic fungus that belongs to the class of Ascomycetes, the fruiting body were formed by the larvae or pupae infection with Cordyceps fungus, it was the same with Cordyceps sinensis, were one of the precious traditional medicines in China. Therefore, artificial culture technology development were be importance, in recent years, it was widely used in the mass production market. In this study, used organic rice to be solid medium and added different concentration of minerals and cyanobacteria to cultured as 15、30、45、60 and 75 days. Finally, compared the content of Cordyceps fungus, and the appearance was observed. The results showed that Cordyceps militaris cultured with added different concentration of minerals and cyanobacteria. For the concentration of minerals at 100 ppm, the sample cultured as 75 days has the highest content of bioactive compounds, the adenosine, cordycepin, and polysaccharide, content of mycelia were 6.58 ± 0.04 mg/g,0.85 ± 0.01 mg/g, and 255.95 ± 3.74 mg/g, respectively. The adenosine, cordycepin, and polysaccharide, content of fruiting body 43.61 ± 0.10 mg/g,9.87 ± 0.12 mg/g, and 219.95 ± 3.67 mg/g, respectively. For the concentration of cyanobacteria at 100 ppm, the sample cultured as 75 days has the highest content of bioactive compounds, the adenosine, cordycepin, and polysaccharide, content of mycelia were 6.93 ± 0.33 mg/g, 1.02 ± 0.01 mg/g, and 161.2 ± 15.31 mg/g, respectively. The adenosine, cordycepin, and polysaccharide, content of fruiting body were 39.81 ± 0.09 mg/g, 9.20 ± 0.02 mg/g, and 169.45 ± 4.9 mg/g.Therefore the results can know,add with cyanobacteria and minerals applied fungal growth and culture to explore it is worth to explore.
| Identifer | oai:union.ndltd.org:TW/103HKU00255006 |
| Date | January 2015 |
| Creators | Yen Ting Chen, 陳諺廷 |
| Contributors | Yuh Shuen Chen, 陳玉舜 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 67 |
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