碩士 / 國立中興大學 / 食品暨應用生物科技學系所 / 103 / The Lactobacillus plantarum is Gram-positive facultatively anaerobic bacteria, which are common in the intestinal tract of humans. They are important in promoting and maintaining human health. In the present study, using alginate and gelatin by complex coacervation to achieve probiotic encapsulation. Lactobacillus plantarum were encapsulated in alginate—gelatin gels. The main goal of the study was to characterize the resistance of the target strain in alginate-gelatin coacervates processed by freeze-drying, the encapsulate and the release behaviors under simulated gastrointestinal tract conditions to be able to assess the feasibility as an delivery system for probiotics. In addition, the cell viable of freeze dried capsules at room temperature and at 4℃ for eight weeks was detected. We found that coacervated capsules were shown to be more resistance to freeze-drying and simulated gastrointestinal conditions than free ones and single formula, specially under the conditions the ratio of protein and polysaccharide was 4：1. The encapsulation efficiency of alginate–gelatin complex coacervation was achieved 72%. The encapsulated L. plantarum maintained its viability for a longer shelf life at 4℃ than at RT during the storage time.
| Identifer | oai:union.ndltd.org:TW/103NCHU5253012 |
| Date | January 2015 |
| Creators | Ru-Li Chen, 陳儒俐 |
| Contributors | Meei-Yn Lin, 林美吟 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 90 |
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