碩士 / 國立中央大學 / 化學學系 / 103 / Brominated flame retardants, such as decabromodiphenyl ether (decaBDE),and hxabromocyclododecane (HBCD), are used to add in the fire protection materials. Comparing to other halogen fire section, they have good flame retardant effect by adding only small amount. Because brominated flame retardants are persistent and highly lipophilic (log Kow> 5), they can be bio-accumulated and bio-magnificated in the food chain. Currently in many human regular consumption of biota products have detected containing such substances.
This study was to develop a simple, effective and rapid sample pretreatment of two brominated flame retardants and apply to different types of biological samples. Experimental extraction method involves the use of vortex-homogenization matrix solid-phase dispersion (VHMSPD) extraction for sample pretreatment, and determination by gas chromatography- electron capture negative ion mass spectrometry (GC-ECNI-MS). The optimal extraction conditions involved dispersing of freeze-dried biota sample (1 g) with 2 g of SiO2 by a vigorous shaking to obtain a homogenous mixture. The mixture was transferred to a glass column containing 1 g 30% acidic SiO2 and 1.5 g SiO2 as the clean-up co-sorbent.The target analytes were then eluted with 20 mL of Hexane: DCM = 1: 1.The extract was transferred to the same column described above, and eluted with 10 mL of the same solvent. The extract was adjusted to 1 μL and then determined by GC-ECNI-MS.
The findings show that the extraction recovery was obtained about 81-108% and relative standard deviations (RSDs) are less than 10 %, respectively. It indicats that the method shows good reproducibility and stability. The detection limit of decaBDE and HBCD were 0.05 ng / g and 0.03 ng / g respectively. In real sample section, decaBDE can be detected between 0.34-2.11 ng / g and HBCD can be dected detween 0.17- 0.5 ng / g in marketed sea food samples.
| Identifer | oai:union.ndltd.org:TW/103NCU05065008 |
| Date | January 2014 |
| Creators | Chun-Chuan Yang, 楊鈞全 |
| Contributors | Wang-Hsien Ding, 丁望賢 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 129 |
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