碩士 / 國立嘉義大學 / 水生生物科學系研究所 / 103 / The aim of this investegation is to evaluate the effects of SDS on survival, growth, lethal D.O., hematology and histology of formosan stripe dace, Candidia barbata, and to quantify the LC50 and safe concentration of SDS to Candidia barbata.
(1) LC50:
Fish (0.11±0.02g) were exposed to 0, 4.2, 4.3, 4.4, 4.5 and 4.6 (ppm) of SDS for 96hr and the survival rate were recorded at 12hr interval. Results indicated the 24, 48, 72, and 96hr LC50 of SDS were 4.42, 4.35, 4.31 and 4.29 (ppm), respectively.
(2) Growth and Feed:
Fish (0.10±0.01g) were reared at 0, 0.0429, 0.0858, 0.429, 0.858 (ppm) of SDS for 8 weeks to grow experiment, after than select the fishes (0.40±0.01g) taken feed experiment. The weight gain (%) and feed rate (%) lower significantly than control, after fish were exposed to 0.429ppm and 0.858ppm of SDS for 8 weeks (p＜0.05).
(3) Lethal D.O., Hematology and Histology :
Three sizes of Fishes (0.09±0.01 g, 0.25±0.01 g and 0.54±0.02 g) were exposed to 0, 0.0429, 0.0858, 0.429, 0.858 and 2.145 (ppm) of SDS for lethal D.O., respectively. Fish (28.74±1.08 g) were exposed to 0, 0.442, 0.884 and 2.21 (ppm) of SDS for 24hr, the blood glucose, lactic acid, hemoglobin, hematocrit and erythrocyte were detected after 0, 12 and 24hr exposure, and gill histological changes was examined after 24hr exposure. It was found that blood glucose concentration, was elevated significantly with increasing SDS concentrations, and the highest value was found in SDS concentration of 2.21 ppm after 12hr exposure (p＜0.05). After 24hr exposure, gill lamellae and terminal nodes hyperplasia lesions was observed, this SDS and lethal D.O. concentration increases with the rise in Vicenza, when the SDS concentration of greater than 0.858ppm, significantly higher than lethal D.O. (p＜0.05).
To conclusion it, When the SDS concentration is greater than 0.858 ppm, the gills lesions was happened, caused hypoxia and stress responses, resulting in blood sugar increased significantly, and increased lethal D.O. And after long-term exposure to SDS, the Candidia barbata feeding rate dropped significantly, leading to weight gain, SGR were significantly decreased, thereby reducing the fry grow! The safe concentration that without influence the growth and physiological responses of Candidia barbata was 0.0858 ppm or below!
| Identifer | oai:union.ndltd.org:TW/103NCYU5061010 |
| Creators | Jian-Wu Lin, 林建吾 |
| Contributors | Shu-Mei Chen, 陳淑美 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 233 |
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