碩士 / 國立宜蘭大學 / 生物技術與動物科學系 / 103 / This study was conducted to investigate the fermented feather meal to replace the high price feed Ingredient of animal diets - fish meal . The experiment is divided into two parts, the manufacture of fermented feather meal and the animal experiments of using fermented feather meal respectively. Feather meal was fermented by Aspergillus oryzae and Bacillus subtilis as fermentation microorganisms. There was 10 g of feather meal to test using different fermentation period, temperature, inoculums volume of microorganism, pH of the solution and moisture percent in initial fermentation conditions test. After that, adding different carbon and nitrogen sources and other nutrient source to stimulate the microorganism growth and enhance the activity of the microbial keratinase. According to the results, the incubate period and the moisture are most effective of enzyme activity of the feather meal fermentation. And there was some feather processing going to use high temperature high pressure to be treated in the beginning. So we increase the incubate period, moisture content and sterilizing time for testing. The results show that the optimum fermentation conditions of Aspergillus oryzae was: incubate period of 10 days, the moisture content of 350%, the solution pH 8, inoculums volume of microorganism 1 mL/10 g feather meal, the culture temperature is 37℃ and sterilized for 40 minutes. The optimum fermentation conditions of Bacillus subtilis was : incubate period of 10 days, the moisture content of 350%, the solution pH 8, inoculums volume of microorganism 2 mL/10 g feather meal, the culture temperature is 37℃ and sterilization time of 30 minutes. The pepsin digestibility of both two microorganism degraded feather meal are more than 60%. The second part of the test animals was chosen the mule duck as experimental animals. And using the Bacillus subtilis fermented feather meal to replace 0%, 25%, 50%, 75% and 100% fish meal of the diet. The results showed that Bacillus subtilis fermented feather meal can be completely replaced all fishmeal of the diet. There's no significantly adverse effect on the growth performance and parameters of blood characters of mule duck.
| Identifer | oai:union.ndltd.org:TW/103NIU00289022 |
| Date | January 2015 |
| Creators | Po-Hsin Huang, 黃柏鑫 |
| Contributors | Shih-Che Huang, 黃士哲 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 61 |
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