碩士 / 國立高雄海洋科技大學 / 水產養殖研究所 / 103 / The aims of this study were to comprehend the roles of Estradiol-17β (E2) in the brain of yellowfin porgy (Acanthopagrus latus) during vitellogenesis and at the final oocyte maturation (FOM). We analyzed the expression levels of brain ERs, CYP19a1b, GnRHs, the expression levels of pituitary FSHβ and LHβ after treated with LHRH-A, E2 and HCG in one-year-old yellowfin porgy during non-spawning season; and examined the egg quality, the distribution of oocytes diameters, the expression levels of ERs, CYP19a1b, GnRHs, FSHβ and LHβ in the brain or pituitary of the matured two-year-old female fish after LHRH-A, HCG, E2 and ATD treatments.
According to the results of the study and our previous studies on yellowfin porgy, we speculated that GTHs could stimulate the expression levels of brain ERα, ERβ1, and CYP19a1b may due to binding with the GTH receptors which were presented in the brain, and then E2 which was syntheses in the brain, interacted with ERα and stimulated vitellogenesis. Our study showed that sbGnRH involved of the regulation of FOM of yellowfin porgy. During the FOM, the brain E2 and GTHs simultaneously stimulated the expression of brain ERs; the expression of brain ERα might be involved in the regulation of yolk formation and the FOM; the expressions of brain ERβ1and ERβ2 might also regulate the FOM; the expression of brain GPER might be relate to inhibited GVBD in full-grown stage oocytes.
The treatment of HCG combined with E2 in fish could increase the ration of full growth oocyte in ovary, and as the result in increased the amount of ovulated oocyte in following stimulation. Pretreatment of HCG combined with E2, then induced FOM with LHRH-A and decease endogenous E2 at the same time, could improve egg quality and get success in spawning. Our study could provide the application for reproduction of multiple spawning fish, which were low quantity of oocyte in each batch of spawn, and difficult in multiple spawn in artificial environment.
| Identifer | oai:union.ndltd.org:TW/103NKIM0086007 |
| Date | January 2015 |
| Creators | Jeng-Ru Wu, 吳政儒 |
| Contributors | Shan-Ru Jeng, 鄭絢如 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 100 |
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