碩士 / 國立屏東科技大學 / 動物疫苗科技研究所 / 103 / Toxoplasma gondii, an obligate intracellular protozoan parasite, causes toxoplasmosis in a broad range of warm-blooded animals. Toxoplasmosis often causes abortions and fetal damage in livestock and pregnant women. The animals are infected by eating infected meat, by ingestion of feces of an infected cat, or by transmission from mother to fetus. Currently, only one T. gondii commercial vaccine is available for livestock, and only partial protection in few animals. Therefore the aim of this study is development of long-lasting T. gondii vaccine. Recently, some research aim to use chitosan in vaccine for protein delivery, and sodium alginate can increase stability of chitosan microparticles. In this study, bovine serum albumin (BSA) was used as initial material for determining the parameters of chitosan/sodium alginate microparticles, such as the concentration of sodium alginate and chitosan in microparticles production, the proportion of protein and microparticles in encapsulation, and the time and environment for release, etc.. Then immunogenicity of the BSA-coated microparticles was analyzed by animal test and some immune assays. T. gondii protein was used as antigen in secondary animal test that following the conditions in initial test. The total IgG, IgA, T-cell proliferation and challenge assay were analyzed. Although this research could not get promising results yet, we hope the following approach can achieve the aim of long-term releasing to stimulate the immune system and then to protect animal against T. gondii infection.
| Identifer | oai:union.ndltd.org:TW/103NPUS5290010 |
| Date | January 2015 |
| Creators | Ye, Shou-Hung, 葉首宏 |
| Contributors | Chung, Yao-Chi, 鍾曜吉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 61 |
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