博士 / 國立中山大學 / 海洋科學系研究所 / 103 / Most of antimicrobial peptides (AMPs) are less than 10 kDa in molecular weight, hydrophobic, membrane active, and have an overall net positive charge. To date, few AMPs have been isolated from marine diatoms. This research studied novel AMPs from marine benthic diatoms and examined their antimicrobial activity. Extracellular extracts of the benthic diatom Nitzschia sp. 1 CCL-2013 were analyzed; the major fraction was less than 6.5 kDa of molecular weight. LC-MS/MS analysis on this major fraction identified 336 peptides. Out of the 336 peptides, 6 potential AMPs were identified using the following strigent critera: being 10 to 15 aa in length, having more than positive one to three in total net charge and at percentage of hydrophobic residues between 0.53 and 0.6. One peptide which was named as ABMOK1 was arbitrarily picked from the 6 peptides and tested for its anbitiotic activity. This novel AMP, ABMOK1, is characterized by the sequence of CGYCGACVGVCK. The SEM of images of Escherichia coli treated by 100 g/mL of ABMOK1 showed that holes were punched on the bacterial membrane, suggesting that ABMOK1 is a membrane-active antibacterial peptide which takes a detergent-like mode of destructive action.
| Identifer | oai:union.ndltd.org:TW/103NSYS5276007 |
| Date | January 2015 |
| Creators | Chiu-chin Lu, 呂秋錦 |
| Contributors | Hin-Kiu Mok, Houng-Yung Chen., 莫顯蕎, 陳宏遠 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 110 |
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