碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 103 / The pathophysiology of spinal cord injury(SCI)involves primary and secondary mechanisms of injury, most research however has focused on understanding the pathophysiology of the secondary damage processes and reducing the amount of delayed cell loss following SCI. The pathophysiological hallmarks of secondary injury processes include the inflammatory response, reactive oxygen species or free radical generated, and neuron apoptosis. SCI-induced reactive oxygen species(ROS)generated were elicits oxidative damage, and promoted the inflammatory response. In present study we examined the anti-inflammatory response and anti-oxidative damage effects of WY8 in rat SCI model. The spinal cord contusion injury was induced in thoracic spinal cord of in female Wistar rats by NYU impactor. The saline, WY8(10 μg), and methylprednisolone(MP; a standard SCI agent 30 mg/kg)was administered by intrathecal injection for 7 days and intraperitoneal once after SCI respectively. We found that intrathecal injection WY8 were keep the spared white matter area, reduced the lesion area, and significantly upregulation locomotor function after SCI. Immunohistochemistical analyses showed that WY8 were significantly attenuated SCI-induced superoxide (O2-), including generating enzyme NADPH oxidase (NOX2), byproduct of NO-O2- nitrotyrosine (NT), and inflammatory mediators such as inducible nitric oxide synthase (iNOS), intercellular adhesion molecule-1 (ICAM-1), interleukin-1beta (IL-1β), and tumor necrosis factor-alpha (TNF-α) expression. In conclusion, WY8 is a potential candidate compound for drug development to treat SCI.
| Identifer | oai:union.ndltd.org:TW/103NSYS5277011 |
| Date | January 2015 |
| Creators | Chen-Yu Yang, 楊振宇 |
| Contributors | Zhi-hong, Wen, 溫志宏 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 171 |
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