碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 103 / In order to discover bioactive secondary metabolites, we have studied the chemical constituents from the organic extracts of red algae Laurencia tristicha. This study resulted the isolation of nineteen compounds, including eight new chamigrane-type sesquiterpenes 1–8 and one new cuparane-type sesquiterpene 9 along with nine known compounds. Among them, compound 2 possess new skeleton.The structures of isolated were determined on the basis of extensive spectroscopic analyses (IR, MS, 1D and 2D NMR) and comparison of spectral data with those of related known compounds. The relative configuration of compound 12 was further supported by a single-crystal X-ray diffraction analysis. In addition compound 10 and 11 were isolation for the first time from natural sources.
The cytotoxicity of compounds 1–19 against the A549 (human lung epithelial cell line), DLD-1 (human colon adenocarcinoma), and LN-Cap (human prostate cancer cells) tumor cell lines were determined. The results showd that compound 13 exhibited cytotoxicity against the A549 cell line with an IC50 value of 16.0 ± 3.3 μg/mL. In anti-inflammation assay compounds 15 and 16 displayed strong inhibition of superoxide anion generation and elastase release, compounds 3、6、13 and 18 show strong inhibition of superoxide anion generation and compound 17 displayed strong inhibition of elastase release. Furthermore, compounds 12, 14 and 17 showed antibacterial activity against Enterobacter aerogenes (ATCC13048), compounds 3, 11, 14, 15 and 17 showed activity against Serratia marcescens (ATCC25419), and compounds 3, 14, 15 and 17 showed activity against Yersinia enterocolitica (ATCC23715).
| Identifer | oai:union.ndltd.org:TW/103NSYS5277021 |
| Date | January 2015 |
| Creators | Jia-yu Chen, 陳嘉妤 |
| Contributors | Jhy-Horng Sheu, 許志宏 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 201 |
Page generated in 0.193 seconds