碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 103 / I examined the role and function of mitochondrial manganese superoxide dismutase (mtMnSOD) in the immune response of white shrimp Litopenaeus vannamei through RNA interference (RNAi) and the genomic organization by genome walking. The cDNA full lengh of white shrimp mtMnSOD is 1193 bp (KP099968) and genomic organization of mtMnSOD consists of two exons (359 bp and 834 bp) separated with one intron (1947 bp). The 5’ upstream of mtMnSOD is 2394 bp and there are lots of transcription factor binding sites including activator protein-1 (AP-1), activator protein-2 (AP-2), specificity protein 1 (Sp1), CAAT-enhancer binding protein (C/EBP), cyclic AMP response element (CRE), GATA-1, nuclear factor-κB (NF-κB), interferon regulatory fator 1 (IRF1), IRF2, IRF3, IRF7A, nuclear factor of activated T cells 1 (NF-AT1), NF-AT2, NF-AT3, and NF-AT4. Among them, Sp1, AP-1, AP-2, and C/EBP play important role in mtMnSOD transcriptional regulation. The mtMnSOD of white shrimp was knocked down in vivo at 6 h post injection with mtMnSOD dsRNA, and superoxide dismutase activity decreased. The phenoloxidase (PO) activity and lysozyme activity increased significantly, but no significant difference in hyaline cell counts, granular cell counts, total hemocyte counts, and respiratory burst after mtMnSOD silencing were observed. The expressions of integrin β, GPx, mtMnSOD, proPO I, proPO II, α2-macroglobulin, and PPA decreased significantly. However, no significant difference in the expressions of LGBP, PX, lysozyme, HSP 70 was observed. Reduction in integrin β reduces ROS production during phagocytosis after mtMnSOD silencing. Increase in PO activity and lysozyme activity are considered to compensate immune system in mtMnSOD silencing.
| Identifer | oai:union.ndltd.org:TW/103NTOU5086002 |
| Date | January 2015 |
| Creators | Chang, Yu-Hsuan, 張瑜軒 |
| Contributors | Gong, Hong-Yi, Chen, Jiann-Chu, 龔紘毅, 陳建初 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 73 |
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