碩士 / 國立臺灣海洋大學 / 食品科學系 / 103 / Sulfated polysaccharides is mainly composed of sulfate and also has anti-inflammatory activity. Chitosan, a natural polysaccharide, has many advantages, such as non-toxic, non-allergenic, biocompatibility, biodegradability and so on. Chitosan has been used as a wound dressing with hemostatic properties, antibacterial and antimicrobial activity, and it can promote wound healing and reduce scarring. In this study, we prepare sulfated polysaccharide/chitosan composite films and evaluated the composite films for the physical and chemical characteristics, blood clotting properties, anti-inflammatory and wound healing. The experimental results show that the sulfated polysaccharide/chitosan composite films have better swelling, mechanical properties and adequate water vapor transmission rates. In vitro studies show that sulfated polysaccharide/chitosan composite films exhibited non-toxic effects on L929 and HaCaT cells and cells can attached on the films by extending pseudopodia. Sulfated polysaccharide/chitosan composite films have blood clotting effect. Anti-inflammatory tests show that both sulfated polysaccharide and Sulfated polysaccharide/chitosan composite films can reduce the secretion of NO and TNF-α, the inflammatory response slowed. Animal experiments showed that sulfated polysaccharides/chitosan composite film can promote wound healing in the 3rd, 5th and 7th, and accelerated angiogenesis and collagen protein production in the 10th, 14th and 21th. This results suggested that the sulfated polysaccharide/chitosan composite film have good swelling, cell compatibility, promote blood clotting and accelerate wound healing effect. It could be applied as a potential material of wound dressing.
| Identifer | oai:union.ndltd.org:TW/103NTOU5253075 |
| Date | January 2015 |
| Creators | Lin, Ying-Ru, 林映汝 |
| Contributors | Huang, Yi-Cheng, 黃意真 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 64 |
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