碩士 / 國立臺灣海洋大學 / 海洋生物研究所 / 103 / This research is to investigate the change of microbial communities after the double stresses of UV radiation and oil contamination. In the past, our lab had collected sediment and seawater samples from the oil polluted coast and prepared the UV resistant diesel oil degrading enrichment cultures, UV1, UV12 and UV24 by adding 1% diesel oil to the sediment culture that had been exposed for 1, 12 and 24 hours to UV(A/B) radiation. The control group, Ori, was an enrichment culture that only added with diesel oil. In order to know the exact role of bacteria and yeast in these enrichment cultures in oil degradation, pyrosequencing, flow cytometry (FCM), real-time PCR (Q-PCR) and scanning electron microscopy (SEM) were used. Bacterial or fungal inhibitor was added to these UV resistant enrichment cultures to investigate the role of bacteria or fungi in degrading the hydrocarbons. The results of pyrosequencing analysis showed that the majority of bacterial communities were Bacteroidetes and Proteobacteria in group Ori and UV1 while in UV12 and UV24 were Firmicutes. Diversity of bacterial communities in these enrichment cultures decreased sharply along with the UV1, UV12 and UV24. We found that in enrichment culture UV12, amending with fungal inhibitor had better oil degrading activity than that amending with bacterial inhibitor. On the other hand, in enrichment culture UV24, amending with bacterial inhibitor had slightly better oil-degrading ability than that amending with fungal inhibitor. The results of real-time PCR showed that bacteria were the majority in both UV12 and UV24 communities.
| Identifer | oai:union.ndltd.org:TW/103NTOU5270001 |
| Date | January 2015 |
| Creators | Lin, Kun Ting, 林坤廷 |
| Contributors | Liu, Shiu-Mei, 劉秀美 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 75 |
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