博士 / 國立臺灣海洋大學 / 海洋生物研究所 / 103 / Attempts were first made to identify the possible causative agents and species in a fishing shrimp poisoning case occurred in Keelung, North Taiwan, 2006. The patient’s symptoms were more similar to those of illegal food additive boric acid poisoning or those of legal food additive sulfite poisoning. To establish the species identification of poisoned shrimp sample, 10 kinds of commercial shrimp species were collected, identified species by judging for outside appearance phylogency, and then used polymerase chain reaction (PCR) to amplify the partial mitochondrial cytochrome b (Cyt b) gene. Then the 401 to 407-bp fragment of the cytochrome b gene was amplified from the shrimp meat by using the primer pair UCYTB151F/270R, and the complete information of the partial gene sequence after gene sequence analysis was obtained. Furthermore, the PCR products were digested by using the endonucleases Alu I, Ssp I and Hae III and analyzed by SDS-PAGE electrophoresis. The electrophoresis profiles of 10 shrimp species could be successfully differentiated. Meanwhile, poisoning patient’s vomitus was amplified by PCR method and analyzed by gene sequencing and restriction fragment length polymorphism (RFLP), showing the species in the vomited shrimp meat sample as rose shrimp (Parapenaueus fissuroides). Moreover, the commercial shrimp meats were identified giant tiger prawn (P. monodon), rose shrimp (P. fissureoides) and white shrimp (Litopenaeus vannamei). In addition, the commercial fishing bait shrimp species were identified mainly rose shrimp (P. fissureoides) and white shrimp (Litopenaeus vannamei).
Secondly, in order to investigate the toxic substances of poisoning patient’s vomitus, which analyzed borax (caculated by boric acid) and sulfite (caculated by sulfur dioxide) content of vomitus. Meanwhile, the content of boric acid and sulfur dioxide in poisoned bait shrimps from fishing shops were determined, indicating boric acid content of vomitus is about 37 mg/g, with trace detection of sulfur dioxide (approximate 0.071 mg/g). Furthermore, the boric acid content of other fishing bait shrimps from fishing shops was 1.4-3.8 mg/g, with the trace of sulfur dioxide content was 0.072-0.085 mg/g. However, the boric acid was not detected (＜0.001 mg/g) in commercial shrimp products sold in the common markets, but the trace sulfur dioxide was detected (0.076-0.088 mg/g).
Moreover, the causative species and agent of sunfish caused another food poisoning incident in Hualien in 2005 were also studied. By using PCR-RFLP method, the species of poisoned sunfish is identified as Masturus lanceolatus. The causative agent of poisoned sunfish is identified as polytoxin-like compound by judging the characterics of clinical symptoms of patients and hemolysis.
| Identifer | oai:union.ndltd.org:TW/103NTOU5270009 |
| Date | January 2015 |
| Creators | Huang, Ken-Meng, 黃耿盟 |
| Contributors | Liu, Shiu-Mei, 劉秀美 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 90 |
Page generated in 0.0016 seconds