碩士 / 國立臺灣海洋大學 / 環境生物與漁業科學學系 / 103 / There are 8 genus and around 90 species of Pomacanthidae in the world. The Royal angelfish or regal angelfish, Pygoplites diacanthus, is a species of marine angelfish of the family Pomacanthidae, and the monotypic genus Pygoplites. It was found in tropical Indo-Pacific oceans, ranged from East Africa, Red Sea, east to French Polynesia Tuamotu Archipelago, north to Japan and south to Australia. This species is popular in aquarium trade because of its colorful body patterns. The Royal angelfish in Indian and Pacific Oceans have has different color morph, The Indian Royal angelfish has an orange face; while Pacific one has a grey face. Whether the Royal angelfish in different Ocean represent different populations or different species is still unclear. The establishment of mitogenome and the analysis of DNA barcode may help to resolve the questions of population genetics, molecular phylogenetics, and evolution for Royal angelfishes. Firstly, we used Next Generation Sequencing (NGS) for the short reads sequencing to decode the mitogenome of Royal angelfish from Pacific (Indonesia) and Indian Ocean (Maldives). The mitogenome of Pacific Royal angelfish was totally 16,784 bp, including 13 protein coding genes, 22 transfer RNAs, 2 ribosomal RNAs and one control region; while the mitogenome of Indian Ocean Royal angelfish was 16,767 bp.The nucleotides composition is similar between two geminate species, including 28.5% A, 28.9%C, 16.3%G and 26.3%T. The mitogenome difference between these two morph types is about 1.55%. The most different region was 10% for control region, then 1.73% for ND6. The analyses of life barcode COI suggested that Royal angelfishes can be divided was into two groups. The estimated divergence time based on the molecular clock concept was about 0.5 My, it could because of the low sea level during Pleistocene ice ages, the emerge of the Indo-Australian Archipelago may form vicariance which direct to the divergence of Royal angelfish in different Oceans and led to the formation of many geminated species in Indo-Pacific.
| Identifer | oai:union.ndltd.org:TW/103NTOU5451014 |
| Date | January 2015 |
| Creators | Chao, Shu-Chen, 趙淑貞 |
| Contributors | Joung, Shoou-Jeng, Shen, Kang-Ning, 莊守正, 沈康寧 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 50 |
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