碩士 / 國立臺灣科技大學 / 化學工程系 / 103 / Cyanophycin granule polypeptide (CGP) is a non-ribosomal protein synthesis, first found in 1887 BC when Borzi observed the Cyanobacteria under a microscope.
Because the yield rate of CGP is too low by culturing Cyanobacteria, we implant the cyanophycin synthetase gene of Cyanobacteria into Escherichia coli (E. coli) and use Isopropyl β-D-1-thiogalactopyranoside (IPTG) or Lactose induction to increase the yield rate. Nature CGP consist of aspartic acid (Asp) and arginine (Arg) in ratio 1:1, but recombicant CGP consist of Asp, Arg, and lysine (Lys) in ratio 5:2:3.
Due to its mechanical strength CGP is not popular biomaterials. this experiment use water-soluble CGP to graft hyaluronic acid with excellent biocompatibility ,and expect that modification can effectively enhance both the mechanical strength and the growth of cells.
By glutaraldehyde and 1-ethyl-3-(-3-dimethylaminopropyl) carbodiimide(EDC)/N-hydroxysulfosuccinimide(NHS) cross-linking, we use Lys group in CGP to produce HA-CGP 3D scaffold.The 3D scaffold which were observed under a fluorescence microscopy after fluorescence staining were proved that cell could adhesion on this 3D scaffold.
In conclusion, the scaffold could simulate cell growth in vivo, and could help cell growth.
| Identifer | oai:union.ndltd.org:TW/103NTUS5342073 |
| Date | January 2015 |
| Creators | Chien-Che Hung, 洪健哲 |
| Contributors | Wen-Chi Tseng, 曾文祺 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 49 |
Page generated in 0.0015 seconds