碩士 / 國立臺灣科技大學 / 化學工程系 / 103 / Cyanophycin, a non-ribosomal synthesis protein, can be produced by recombinant Escherichia coli. The polymer consists of a polyaspartic acid backbone with arginine and lysine as a side chain. Dextran is a polysaccharide composed by glucose molecules, and is capable of good hydrophilicity and biocompatibility. Dextran sulfate is capable of good biodegradability and it has biocompatible polyanion as heparin. Its structure is a polysaccharide which has negatively charged sulfate groups. In this study, we prepared differnet ratios of cyanophycin - dextran/ cyanophycin - dextran sulfate composite 3D scaffolds by cross-linking reaction with glutaraldehyde. We expect that scaffolds have their properties.
We modified the dextran and dextran sulfate by different method, and then the two polymers which had been modified were grafted with cyanophycin by chemical bonding in order to make more biocompatible polymer. We made the dextran have more aldehyde group in order to make it graft with cyanophycin. We except that the grafting ratio is up to 10%. We also made the dextran sulfate have carboxyl group in order to make it graft with cyanopycin. The graft information of cyanophycin - dextran sulfate was determind by TNBSA. We detected the toxicity of the two polymers (cyanophycin-dextran and cyanophycin dextran sulfate) by the MTT assay. Finally, we mixed cyanophycin-dextran and cyanophycin dextran sulfate in different weight ratio, and then the mixed polymer solution were cross-linked with glutaraldehyde to form 3D scaffold.
The result showed that the cyanophycin-dextran had the grafting ratio about 10%. We confirmed that the cyanophycin-dextran sulfate had grafted with each other. We had information that cyanophycin-dextran and cyanophycin-dextran sulfate was not toxic from MTT assay.
We measured the volume of 3D scaffolds which have different contain of cyanophycin-dextran and cyanophycin-dextran sulfate. The data showed that if cyanophycin-dextran sulfate contains large proportion in the scaffold, the volume will be relatively small. The swelling ratio of scaffolds showed that each scaffold had massive swelling ratio in short time and it also showed that cyanophycin-dextran had more influence than cyanophycin-dextran sulfate on the swelling ratio. The SEM data showed that both surface and cross section had pores.
In conclusion, the scaffold had good hydrophilicity. The scaffolds will be good for cells to migrate because of their proper pore size. We expect that it can use in cell culture.
| Identifer | oai:union.ndltd.org:TW/103NTUS5342076 |
| Date | January 2015 |
| Creators | LIN, YI-SHIN, 林益新 |
| Contributors | Wen-Chi Tseng, 曾文祺 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 81 |
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