碩士 / 國立高雄大學 / 生命科學系碩士班 / 103 / Morinda citrifolia, commonly called as “noni”, is considered to be a tropical fruit with variety of health effects, has been used in folk remedies for over 2000 years. In recent years, some research indicated different components separate from noni fruit, leaf and root have lot of medical benefits. The purpose of this research was to investigate the immunoregulatory effects of polysaccharide extracts from noni leaf on Caridina serrata. In this study, we used hot water to extract noni leaf polysaccharide, and quantified the total sugar by using phenol sulfuric acid method, which allowed us to prepare the amount of immuoregulatory buffer accordingly. In the test of immunity, we used 100 μg/L and 250 μg /L doses of noni leaf polysaccharide respectively as the experimental groups to stimulate Caridina serrata’s immunity, and compared to the groups of one without any immuoregulatory buffer, and one with commercial product (AZOO vitamin). It was taken four weeks to regulate Caridina serrata’s immunity, and then analyzed the immune parameter, adaptability of temperature change and clearance efficiency of D. hansenii to make sure if noni leaf polysaccharide could enhance Caridina serrata’s non-specific immunity or not. The result of immune parameter shows that noni leaf polysaccharide can enhance Caridina serrata’s haemocytes phenoloxidase activity, superoxide anion yield and superoxide dismutase activity. Also, in the aspect of adaptability of temperature change and clearance efficiency indicate that noni leaf polysaccharide can improve the ability of temperature adaptation and clearance efficiency. In conclusion, immersing Caridina serrata in noni leaf polysaccharide can enhance Caridina serrata’s non-specific immunity.
| Identifer | oai:union.ndltd.org:TW/103NUK05105001 |
| Date | January 2015 |
| Creators | Hsuan-en Hung, 洪璿恩 |
| Contributors | Wen-Jen Yang, 楊文仁 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 72 |
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