碩士 / 元智大學 / 生物科技與工程研究所 / 103 / Lutein (β, ε-carotene-3, 3’-diol), a member of the carotenoid group, is a yellow pigment that exists in plants and phototrophic microorganisms like microalgae. Lutein has many functions for the protection of the human’s health. Literatures have proved that lutein can prevent cardiovascular diseases, some types of cancer and age-related macular degeneration. Hence, some local indigenous microalgae were evaluated to select the lutein production algae in our lab. Among the twenty two microalgae strains, Scenedesmus obliquus ESP-5 showed the highest lutein production of 0.10 g/L and lutein content of 1.2 mg/g more than the other microalgae. According to the above mentioned results, this study suggested that Scenedesmus obliquus ESP-5 should be the production algae used. In this study, the effects of the type of the light and intensity of the light and feeding strategies of nitrogen sources on the cell growth and lutein content of Scenedesmus obliquus ESP-5 were investigated. This study successfully developed a lighting strategy that can facilitate the growth of Scenedesmus obliquus ESP-5 and lead to the production of 4.96 mg/L/d lutein at 24 h cultivation (12h light-12h dark). However, the molecular weight and chemical structure of lutein should be identified and characterized in near future.
| Identifer | oai:union.ndltd.org:TW/103YZU05111114 |
| Creators | Yin-Che Hsu, 徐胤哲 |
| Contributors | Yu-Hong Wei, 魏毓宏 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 66 |
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