碩士 / 國立中正大學 / 化學暨生物化學研究所 / 104 / In recent years, there are many research about green algae Chlorella pyrenoidosa and its extracts. How to extract bioactive proteins efficiently from green algae and to know their physic-chemical characteristics play an important role on drug develop and food industry. In this study, we extracted two major proteins from commercial green algae Chlorella pyrenoidosa. After purifying, we analyzed their qualitative properties and investigated their bioactivity on RAW 264.7 cells.
In this research, we used mortar and pestle with extract buffer, fast protein liquid chromatography with different columns and sodium dodecyl sulfate polyacrylamide gel electrophoresis with different staining ways to ensure our proteins were isolated. We successful separated and purified the two major proteins. (15KDa and 30KDa).Using spectra instrument to identify the difference before and after separation was conducted. We deduced that 15KDa protein might have Tryptophan or Valine on its sequence. And the major secondary structure is random coil in solution.
We found that the protein (15KDa) have an activity to induce RAW 264.7 cells for activation and promoted a reaction like LPS but the other protein 30KDa didn’t. In XTT assay there is no cytotoxicity to RAW 264.7 cells and cell viability is upper than 95%.
| Identifer | oai:union.ndltd.org:TW/104CCU00065020 |
| Date | January 2016 |
| Creators | Tsai,Cheng-Shun, 蔡承順 |
| Contributors | Cherng,Jong-Yuh, 程中玉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 93 |
Page generated in 0.0117 seconds