碩士 / 嘉南藥理大學 / 化粧品應用與管理系 / 104 / (PART 1)This study investigated the antioxidantation, antimelanogenesis and DNA repair mechanisms of CYY1P and CYY1F; CYY2, CYY2S, CYY2L in water and ethanol extraction.
The antioxidantation activities were evaluated by measuring activities of free-radical scavenging, total antioxidantation contents, thiobarbituric acid reactive substances and reducing power. Cytotoxicity measurement was demonstrated by keratinocytes (HaCaT cells) and melanoma (B16F10 cells) and we found that the water extract had less cytotoxicity than ethanol extracts. Thus, we accessed the in vivo test by water extracts, such as the reactive oxygen species (ROS), glutathione (GSH), catalase and GPx formation in H2O2-treated in HaCaT cells were tested. The antimelanogenesis assay was analyzed by measuring tyrosinase activities, melanin contents and regulated-protein expressions in B16F10 cells. CYY1PW and CYY2W could downregulated-protein expressions of MITF, MC1R, tyrosinase, TRP-2 and TRP-1. Additionally, the DNA repair mechanism of CYY1PW and CYY2W were studied. These results showed that CYY1PW and CYY2W could promote the HaCaT cells to repair after UVB-irradiated and they increased significantly of protein expression on NER (nucleotide excision repair) system.
These results demonstrated that water extracts of CYY1PW and CYY2W had antioxidandation, antimelanogenesis and DNA repair properties. Thus, the nature products can be used as whitening and repair skincare cosmetics additive.
(PART 2)Betel nut chewing practice is widespread in Taiwan. According to the statistics of the standardized death rate of major cancers by the Health Promotion Administration, Ministry of Health and Welfare, oral cancer is the fifth leading cause of cancer and the number of 6,560 patients grew up to 7,407 patients between year 2010 to 2012. The International Agency for Research on Cancer (IARC) had declared that betel nut with or without additive is carcinogenic to human.
In this study, we investigated the anticancer mechanism of SP3 from marine animal in human oral cancer. The cell cytotoxicity in human head and neck squamous cell carcinoma (SCC4, SCC9 and SCC25), human melanoma cells (A375) and keratinocytes of human gingival squamous carcinoma cell (OECM-1) were determined by MTT assay and the cell autophagy were observed after treatment with SP3 for 48 h. Thus, we also determined the autophagy and apoptosis related protein expression by immunofluorescence, flow cytometer and western blot. Besides, SP3 can increase ROS generation, reduce GSH level and mitochondria membrane potential (MMP) and anti-migration in OECM-1 cell. These results indicated SP3 may cross-link the pathways between autophagy and apoptosis, therefor we suggested that it has potential on oral cancer treatment.
| Identifer | oai:union.ndltd.org:TW/104CNUP0115016 |
| Date | January 2016 |
| Creators | CHEN, YI-YUN, 陳依筠 |
| Contributors | LIANG, CHIA-HUA, 梁家華 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 140 |
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